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Keeping a Laboratory Book

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What is a Lab Notebook? ... Grab the notebooks! For Every Experiment, Record: Start Date on all pages. Title ... Guidelines for Keeping a Laboratory Notebook. ... – PowerPoint PPT presentation

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Title: Keeping a Laboratory Book


1
  • Keeping a Laboratory Book

Abdul Rahman Omar Institute of BioScience Faculty
of Veterinary Medicine Universiti Putra
Malaysia Tel 603-89472102 Fax
603-89472101 E-mail aro_at_ibs.upm.edu.my
2
What is a Lab Book?
  • Complete record of procedures, reagents, data,
    and thoughts to pass on to other researchers
  • Why experiments were initiated, how performed,
    and results, comments
  • Place to compile data/charts/photos/ideas
  • Place of clues, to troubleshoot problems
  • Place to observe whole picture and think
  • Legal document, to prove patents
  • Defense against accusations of fraud or lawsuits

3
Type and Format
  • Ultimately dictated by your project
    leader/company

4
Physical Characteristics of a Good Lab Book
  • Large, 8.5x11 at least (attaching stuff)
  • Bound (stitched) pages to ensure integrity
  • Numbered pages
  • White gridded
  • Acid free paper (30 years)
  • Written in Pen (gel pens are good).

5
http//www.swarthmore.edu/NatSci/cpurrin1/images/p
enexp2.jpg
6
How Important?
  • In case of Lab Fire
  • Grab the Lab Books!

7
For Every Experiment, Record
  • Start Date on all pages
  • Title
  • Why Brief statement of purpose
  • How
  • Protocol with reference of origin, calculations
  • What Happened
  • All that happens (protocol changes)
  • Taped in Information or keep an associated
    folder
  • What It Means
  • Your interpretation (summation with oddities and
    comments)
  • Whats Next

8
Attached Materials
  • Computer generated data
  • Photographic data
  • Printed graphs (make as you go)
  • Datasheet templates
  • Product labels
  • Who provided plasmids, etc.
  • Notes (or pasted copies) of discussions,
    conversations, emails, readings related to expt
    design or goals
  • Archive locations of plasmids, probes, etc.
  • X-rays and other large items may be kept in a
    separate folder if they dont fit in the lab
    notebook.
  • Always write on these materials the date and
    other identifying information in case they get
    separated!

9
Frequently Forgotten Information
  • Serum lot number
  • Antibody titer
  • Other people involved
  • Centrifuge model, speed, temp
  • Incubation time
  • washes
  • Tube size and type
  • Unexpected delays
  • Growth medium used
  • Buffer pH
  • Calculations
  • Initial cells
  • Age/passage culture
  • Gel
  • Growth stage Bact.
  • Condition of cells used

10
Characteristics Helpful for Filing a Patent
  • In US, patents on intellectual property are
    awarded not by the first to file, but first to
    invent first to record! But...
  • In EU, patent belongs to first to file
  • Sign and date each entry
  • Have an independent witness sign and date each
    entry
  • Do not change entries (make a new entry
    cross-reference to prior entry)
  • Use past tense
  • Never remove original pages or attachments
  • Record all discussions/meetings/ideas relevant to
    the project
  • Record as much detail as possible
  • http//www.invention-protection.com/ip/publication
    s/docs/A_Primer_On_Lab_Notebooks.html

11
The importance of timing
  • Always record, update, review
  • Record as you go
  • Input paper towel and post-it info ASAP!
  • At the LATEST, insert data the next day!
  • Do a weekly checkup
  • 1 hour to review
  • Make sure everything is attached securely, all
    summaries written, future directions written,
    record in table of contents

12
Tips to Preserve Data Integrity
  • Never, ever, remove a page
  • Fill consecutive pages
  • Cross out unused parts of pages
  • Record all info as accurately as possible.
  • Do NOT omit any result, no matter how odd.
  • Cross out mistakes lightly (might need to
    recover)
  • Write legibly
  • Put a full date (international date problems)
    with month spelled out.

13
(No Transcript)
14
Taken from Linus Pauling Research Notebooks,
http//osulibrary.orst.edu/specialcollections/rn
b/index.html
15
References
  • Kathy Barker, At the Bench A laboratory
    Navigator. Cold Spring Harbor Laboratory press.
    1998.
  • Guidelines for Keeping a Laboratory Record.
    David Caprette, Rice University.
    http//www.ruf.rice.edu/bioslabs/tools/notebook/n
    otebook.html
  • Guidelines for Keeping a Laboratory Notebook.
    Colin Purrington, Swarthmore Univ.
    http//www.swarthmore.edu/NatSci/cpurrin1/notebook
    advice.htm
  • Laboratory Record Keeping. Todd E. Garabedian,
    Nature Biotechnology v. 15 (August 1997)
    pp.799-800http//biotech.about.com/gi/dynamic/off
    site.htm?sitehttp3A2F2Fwww.wiggin.com2Fpubs2
    Farticles_template.asp3FID3D102187242000
  • Office of Research Integrity, U.S. Dept. of
    Health and Human Services http//www.unh.edu/rcr/
  • Responsible Conduct of Research Online Study
    Guide. Julie Simpson, University of New
    Hampshire http//www.unh.edu/rcr/
  • Francis L. Macrina, Scientific Integrity An
    Introductory Text with Cases. ASM Press. 2000.

16
Guidelines For The Use of Laboratory Book
Laboratory Book Universiti Putra Malaysia
17
Why need to keep Lab Book
  • To maintain and continuously improve the quality
    of research
  • MS ISO90012008 system requirement
  • The lab book has been designed to address the
    requirement of design and development of research
    (MS ISO90012008 clause 7.3)
  • Monitoring of research progress by different
    processes
  • Review
  • Verification
  • Validation (in some research that involved
    validation of prototypes, software, modules, etc)

18
Keeping Laboratory Book
  • Laboratory Book Format
  • Some examples
  • http//www.ibs.upm.edu.my/ibsbi/note.html

19
  • Thank You
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