Diapositive 1

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RNA editing of non-NMDA receptor subunits
M1 M2 M3 M4
COOH
NH2
Q R

• Glutamine (Q) - unedited
• (GluR1, 3, 4)
• high Ca2 permeability
• inwardly rectifying I/V
• high single-channel conductance
• blocked by intracellular spermine

• Arginine (R) - edited
• (GluR2)
• low Ca2 permeability
• linear I/V
• low single-channel conductance
• not blocked by intracell. spermine

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NR3A NR3B
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Fast glutamate concentration jump
Glutamate glycine
piezo
Glycine alone
Patch-pipette
1 ms pulse of 1 mM glutamate
10 pA
500 ms
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Fast glutamate concentration jump
piezo
Patch-pipette
1 ms pulse of 1 mM glutamate
10 pA
500 ms
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Fast glutamate concentration jump
Glutamate glycine
piezo
Glycine alone
Patch-pipette
1 ms pulse of 1 mM glutamate
10 pA
500 ms
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NMDA channels following a 1 ms pulse of 1 mM
glutamate show characteristic NR2D properties
34 pS
100
75
Number
50
21 pS
25
0
0
1
2
3
Amplitude (-pA)
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Single NMDA receptor channel events
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NMDAR-channels in Golgi cells
wild-type
NR2D-knockout
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EM CA1 dendrite
Comparative Morphology of Dendritic Spine
Synapses Hyppocamp, CA1 stratum radiatum
Excitatory synapses are shown in red. Inhibitory
synapses in blue. The dendrite has been rendered
transparent in the bottom image to more clearly
show the positions of all the synapses.
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We will discuss today

• Ionic channels
• - general principles of organization
• - how to record channels
• - main functional parameters
• Neuronal plasticity
• - Long-term potentiation
• - Long-term inhibition
• - Short-term modulation.
• Actin cytoskeleton and Ca2 in formation and
  plasticity of spines

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Discovery of Long-Term Potentiation (LTP)
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Number of LTP publications
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