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Greenhouse screening for Fusarium wilt
resistance in lupine N. Kuptsov, J.L.
Christiansen, S. Raza and B. Joernsgaard The
Royal Veterinary and Agricultural University,
Copenhagen Denmark Hoejbakke Alle 5, DK-2630
Taastrup, Denmark, phone [phone], e-mail
bjoatkvl.dk
Archived at http//orgprints.org/00001632
Introduction The interest in growing L.
angustifolius is increasing in humid maritime
areas in northern Europe due to new earlier and
higher yielding varieties. However, these
conditions favor fusarium root rot and fusarium
late wilt development, which could be accentuated
by a short rotation of grain legumes in organic
fields. Thus fusarium resistance is highly
important in these conditions.
Photo 2. Fusarium wilt in L. angustifolius
Materials and methods Susceptible and resistant
genotypes and F2 and F3 populations were grown in
greenhouse in a infection font. The dominant
fusarium species was in descending order F.
oxysporum then F. avenaceum, F. culmorum, F.
solani, F. gibbosum. Where the resistant
genotypes yielded a low frequency of susceptible
scores the segregation ratio in of their F2 and
F3 were corrected for this error (table1).
Results and discussion Large Fusarium wilt
presure was observed and fully susceptible lines
were completely destroyed by this disease caused
by F. oxisporum. Suceptible plants did not formed
pods and had typical stem symptoms. In L.
angustifolius total resistance to wilt was among
others observed in Crystal (Russia), Mitan
(Belarus), Rose, E104, E105, E106 (Denmark),
Tanjil (Australia). In L. albus Giza (Egypt) and
MA (Denmark). All tested L. luteus were resistant
in these conditions, Mortiv-369, WAL-196 etc.
Very susceptible L. angustifolius lines were
Prima (Denmark), Sonet (Poland), Borweta
(Belarus), Kalya (Australia) (photo 1). The
larger part of the L. albus lines was 100
destroyed eg. Lublanch (France), E1 and P1
(Denmark) (photo 2). Single pod descent F2 and
F3 hybrid populations in L. angustifolius showed
that resistant genotypes have two dominant
non-allelic resistance genes to wilt. We call
this gene Relation to Fusarium oxisporum
(Rfo1,Rfo2). Susceptible genotypes have either
two wild genes ( ) or one wild and one
dominant resistant gene ( Rfo2Rfo2 , or
Rfo1Rfo1 ). Crossing Rfo2Rfo2 to
Rfo1Rfo1 segregates in 97 resistant to
susceptible in F2, the same segregation was
observed by crossing Rfo1Rfo1 Rfo2 Rfo2 to
(table 1, fig. 1). Conclusion It is
recommended to use these resistant varieties as a
source of resistance genes for breeding in areas
with potential fusarium wilt problems.

F2
Figure 1. Segregation scheme.
Table 1. Segregation in F2, (Rresistant,
Ssuceptible)
Photo 2. Fusarium wilt in L. albus