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SEPARATION OF DNAS BY CESIUM CHLORIDE DENSITY GRADIENT CENTRIFUGATION ... of radioactivity; an interpretation of the autoradiograph is shown at right. WEAVER: FIG. ...
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Chapter 20 DNA Replication and Repair Replication is bidirectional DNA Replication is Semidiscontinuous Okazaki Fragments DNA Polymerase III is a Multisubunit Enzyme ...
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DNA chains are synthesized 5'- 3' DNA replication is semidiscontinuous ... have to take a detour to make a 0.13 mile Okazaki fragment. and then rejoin the truck ...
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(BCH 101) Nucleic Acids ...
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no de novo initiation of DNA synthesis) 2. require dNTPs ... A: Because DNA polymerases require a primer but can't synthesize them de novo ...
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V. cDNA Libraries (converting mRNA into 'complementary DNA' ... Landmark Experiments in DNA Replication ... Steric Constraints Prevent Catalysis of rNTPs ...
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DNA lacks the ability to perform the process of ... DNA helicase unwinds DNA strands ... DNA topoisomerase relieves mechanical strain induced by unwinding ...
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DNA Replication and Repair Replication is bidirectional E. coli genome size = 4.6 X 106 bp Bacteria have circular chromosome with single origin of replication.
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DNA polymerase has 3' to 5' exonuclease function (epsilon-subunit) that ... Terminator utilization substance (Tus) binds to ter region. ...
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Mix DNA containing nomal isotope of nitrogen(14N) and DNA labeled with a heavy ... Autoradiograph of replicating embryonic Triturus vulgaris DNA ...
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Only have one copy of each gene. Diverse living environments. Evolution studies ... Genetics vs. Biochemistry. Create mutants. Genotypes. Gene function. Purify ...
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DNA Replication and Repair. Watson and Crick Predicted Semi-conservative Replication of DNA ... DNA polymerase III major polymerase involved in DNA replication. ...
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DNA: The Genetic Material A Short history of DNA
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Structure of DNA- key words of polarity, complementary, ... Electron microscopy: Opening figure of Ch. 11. What to know: See the learning objectives! ...
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Chapter 15 DNA Biosynthesis and DNA Damage Repair Section One The General Features of Replication of Chromosomal DNA DNA replicates ...
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Bacteriophage DNA was labeled with radioactive phosphorus (32P) ... only the bacteriophage DNA (as indicated by the 32P) entered the bacteria and ...
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Relieves torsional strain generated by DNA unwinding. DNA gyrase (DNA topoisomerase II) ... the leading strand proceeds as DNA is unwound by the DnaB helicase. ...
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Pol III holoenzyme of E. coli can synthesize hundreds of ... Also most bacteria that cause enteritis: Salmonella, Shigella, E. coli. ...
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Very early it was discovered chromosomes are composed of proteins and DNA. ... Confirmed by Meselson-Stahl Experiment. Replication Process ...
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Hfr bacteria possess F factor integrated into the bacterial genome. ... Human genome project. Animal biotechnology. Plant biotechnology ...
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1. Single type of catalytic subunit (dnaE) used in replication of ... 'Ice-skating' Interactions with DNA via water molecules. Replication of E. coli chromosome ...
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DNA unwinding. Protein recruitment. Priming and DNA synthesis. The functions of initiator ... unwinding. DNA gyrase (DNA topoisomerase) Binds single-strand DNA ...
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Griffith's results: - live S strain cells killed the mice - live R strain cells did not kill the mice - heat-killed S strain cells did not kill the mice ...
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DNA: The Genetic Material Dr. Henry O. Ogedegbe Department of EHMCS The Hammerling Experiment: Cells Store Hereditary Information in the Nucleus Where is hereditary ...
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