EXPERIMENTAL DEMONSTRATION OF SEMIDISCONTINUOUS DNA REPLICATION

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EXPERIMENTAL DEMONSTRATION OF SEMIDISCONTINUOUS
DNA REPLICATION (a) Replicating T4 DNA
pulse-labeled with radioactive precursor for
2-120 seconds (b) When T4 DNA ligase was mutated,
only small fragments of DNA were labeled
WEAVER FIG. 20.7
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DETECTION OF BIDIRECTIONAL DNA REPLICATION DNA
synthesis in Bacillus subtilis spores was
initiated synchronously and labeled for a short
time with a low level of radioactivity and then
pulsed with a high level of radioactivity an
interpretation of the autoradiograph is shown at
right
WEAVER FIG. 20.11
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