Luxr PowerPoint PPT Presentations

LuxR

RNA Polymerase Binding Site Consensus Sequences. 20 bp lux box. What is LuxR / lux? ... lac sequence. b - galactosidase. Results (Fig. 2) ...

luxI/luxR Quorum Sensing Cell-Cell Signalling Receiver + Sender Reporter Target (bead) R OHHL Cell-Cell Signaling: Constructs Sender Receiver Single Cell Construct ...

Lac Operon. pLac, lacI, LacR, IPTG. Lux Operon. pLux, luxI, LuxI, luxR, LuxR, SAM, AHL ... AraC Operon. pAraC, araC, AraC, L-arabinose. Reporters. GFP, RFP, YFP...

Bacterial targeting Fec signal transduction Quorum-sensing luxI/luxR Quorum Sensing Sender + R OHHL Receiver Receivers (luxR + Reporter) GFP Receivers tetR ...

Cross reactivity between 3OC12/LuxR. Time Delayed Growth System. Varied temperature, ampicillin concentration, and agar concentration ...

R0051: Promotor, lambda cI repressed. C0051: cI B0010. B0010. B0012. B0012 ... Promotor activated. by LuxO - P (present. when LuxM is low) Q00400: Tet inverter. LacR ...

yo. sup. Test constructs. luxR. OHHL. BBa_I13263. BBa_T9002. Growth data for ... Construction of GFPmek for PDZ binding. Preparing sLppOmpA for future assembly ...

sigA strong hixL sigA weak hixR hin invertase CinR sigA LuxR LasR rcsA ter---GFP--RBS rcsA-RBS-SigA-LuxR-hixL- SigA- RBS- Hin-terR-terL-hinR-LasR-SigA-RBS-CinR-RBS ...

C'est un homologue de LuxR (25% d 'identit selon l 'alignement donn par ALIGN) ... L 'alignement BabR-LuxR permet de d finir sur la topologie les r sidus importants dans la ...

Bacteria That Contain luxS (A sample of 534 genomes sequenced) Targets of AI-2 ... Quorum Sensing Allows Bacteria To Be Multi-Cellular. Similar to Higher Organisms ...

They cooperate to fabricate complex structure. Information Rich Materials ... Autoinducer fabrication (LuxI) Autoinducer response (Pr ... Molecular Fabrication ...

Rapid growth (E. coli 20 minutes per generation; therefore, 1 ... Bioluminescence in Vibrio fischeri. LuxS/AI-2 quorum-sensing system. Quorum sensing in EHEC ...

Device output is PoPS produced at a LuxR-regulated operator. Usage ... flask, supplemented M9 media, FACScan cytometer [see MIT SBWG FACS protocol] ...

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V. harveyi Reporter Strain AI-2. Quorum Sensing in E. coli and Salmonella ... Actinobacillus suis. Bacillus anthracis. Bacillus halodurans. Bacillus subtilis ...

To develop a new class of. broad spectrum antibiotic. based on bacterial. quorum sensing. ... Antibiotic Cellular Targets. Density regulated gene products. Y ...

Further plans: readjust part to BioBrick standard. Combine part BBa_F1610 and BBa_I0500 (pBAD promotor) Possibly: clone CFP cassette behind this new sender part ...

Density Dependent Bioluminescence. free living, 10 cells/liter 0.8 ... Expressed in E. coli DH5a showed bioluminescence. Experimental Setup. BIO-TEK FL600 ...

Time (min) 0. 30. 60. 90. 120. 150. Relative Concentration. 0% 100%. KatE degradation lags behind GOase production. 4. Parts Breakdown ...

Engineer coordinated behavior using diffusion-based ... Rhizome interactions. rhiI. rhiR. Pseudomonas aureofaciens. Plant beneficial. Phenazine production ...

Physiological Testing of Host Pathogens in an E. coli Specific Bioreporter System ... North Carolina State University. July 12, 2004. Kathleen Daumer, Principal ...

Lingchong You, Robert Sidney Cox III, Ron Weiss & Frances H. Arnold ... Presented by Victoria Hsiao. What They Did. They built and characterized a 'population ...

Bacterial biofilms are impermeable to antimicrobial agents ... MRSA strains are associated with nosocomial infection this is a potential future application ...

Finding new targets with signaling. Random library. Computational Approach ... One-cell/two-cell quorum systems. Characterized. Targeted quorum senders ...

iGEM @ Imperial Starting off: Week 1 Week 1 Engineering/Biology Introduction Lectures Journal Club Wiki Brainstorming 3 ideas Only three ideas made the cut.

Only other step requiring work is actual stamping. Still not very precise; can we blot? ... circuit design. Finish building Las parts with new reporters ...

... bacteria in the surrounding and trigger the increase in luminescence. The gene that regulates the luminescence is called lux. See paper (R10) on the website. ...

Only three ideas. made the cut. Bio-Clock (Re-defining Time) ... Very High Compression due to small size of bacteria. The Oscillator ...

Biofilm and Quarum Sensing M. Sc (P) Biotechnology * * * * * Autoinducer peptides cont

BioLogic We re going to use The Quorum System Small RNAs 2-Hybrid systems (and a 3-Hybrid system)

Quorum sensing and multidrug transporters in Escherichia coli PNAS, 2006, 103(7): 2386 2391 Shirley Yang, Christopher R. Lopez, and E. Lynn Zechiedrich

Cellular Computation and Communications using Engineered Genetic Regulatory Networks

This presented indicates that how microbial organism are communication from one cells to another cells.

sociomicrobiology Biofilms Quorum sensing Biofilms Community of microbes embedded in an organic polymer matrix that adheres to a surface 3D structure ...

To develop a system for directing bacteria to a target of interest ... in collaboration with the lab of Costas Maranas, Penn State Dept of Chemical Engineering. ...

Quorum sensing in P. aeruginosa Quorum sensing in P. aeruginosa cont.. Inhibition of quorum sensing What is the need for Quorum sensing inhibitors ?

'Community of microbes embedded in an organic polymer matrix that adheres to a surface' ... Synthetic halogenated furanones abrogate pneumonia in mouse model of acute ...

If used in a cell containing TetR then a second input signal such as aTc can be ... Author(s): Barry Canton [bcanton@mit.edu], Anna Labno [labnoa@mit.edu] Last ...

Brown iGEM. international genetically engineered machines competition. July Update ... Most kinks worked out of the way. First ligations completed ...

... Standard Parts Parts should not have multiple functions (One subunit of T7 phage DNA polymerase is actually E. coli thioredoxin) ...

The intended signaling molecule is 3OC6HSL. ... RNAP/copy - ? Compiled By - Barry Canton (bcanton@mit.edu) Last Updated - 5/9/05 ...

Bacterial Quorum Sensing Quorum Sensing Is Central to Symbiosis Quorum Sensing: Population Density-Dependent Gene Regulation Quorum Sensing: Population Density ...

Construction Intermediates. Cell-Cell Signaling Constructs. Receiver. Sender ... Intercellular signaling: QS. Intracellular signaling: Fec. ACKNOWLEDGEMENTS. Advisors ...

Bacterial targeting is necessary for spatially-specific activity ... in collaboration with the lab of Costas Maranas, Penn State Dept of Chemical Engineering. ...

Charles Darwin, immortalized in E.coli. Projects mentioned in the ... Toning Down C1 ... sites (RBS) to the gene sequence, they were able to tone down ...

Mic 428 - Lecture# 17 - Regulation of Gene Expression. Other global control networks: Quorum ... Nature Reviews/ Microbiology. 581-592. Autoinducer detection ...

In our model system, luminescence will be induced by the presence of both ... into Pseudomonas fluorescens where the system will be tested for luminescence. ...

Protein Trafficking & Cell-cell communications. Criticisms & Conclusion. Translation. Interpreting the information coded in the mRNA into proteins ...

Determined that our YFP reporter (used in the receiver) is defective. Re-ordered a working YFP and began ... Parts are being rebuilt with new reporters anyways ...

Example 1 - results. Treating cells with UV ... The properties of the output is determined by the characteristic of the network ...

BIO FILMS AND QUORUM SENSING , SOCIAL MICROBIOLOGY

Fluorescence levels at different concentrations of AHL ... Higher sender cell concentration. Creating a time course for AHL induction with receiver construct ...

Advisors: Professors Adam Arkin and Jay Keasling. GSIs: Chris Anderson and John Dueber ... Samantha Liang. Bryan Hernandez. Kaitlin A. Davis. Daniel Kluesing ...

Transformation of Escherichia coli Using an Inducible Expression Vector Containing the Bioluminescent Vibrio fischeri Lux Operon by Bryan Hart & Crystal Harmon

If used in a cell containing TetR then a second input signal such as ... Barry Canton [bcanton@mit.edu] Anna Labno [labnoa@mit.edu] Last Update: 5 October 2006 ...