Cling-E. coli

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Cling-E. coli

• Lets get together Quorum-sensing
• Stephanie Lo, Perry Tsai
• July 9, 2007

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Random peptide display and screening
LppOmpA
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Bringing bacteria together
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Test constructs
BBa_I13263
BBa_T9002
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Growth data for logphase determination
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Fluorescence response to OHHL induction
GFP
YFP
100nM OHHL
10nM OHHL
Non-induced No luxR
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BioBricks, assemble!
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Looking into new BioBricks
LuxI amplifier
LuxR generators with different promoters
LuxI amplifier and reporter
LuxI receiver with different reporter
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In other LppOmpA news Lpp-OmpA PDZ Western
blot
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Synthesized LppOmpA (sLO)
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This weeks agenda

• FACS appointment on July 11
• Checking out ligations and new BioBricks
• More fluorescence assays, possibly with new
  constructs
• Construction of GFPmek for PDZ binding
• Preparing sLppOmpA for future assembly