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LOGO

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Only three ideas. made the cut. Bio-Clock (Re-defining Time) ... Very High Compression due to small size of bacteria. The Oscillator ... – PowerPoint PPT presentation

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Title: LOGO


1
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2
iGEM _at_ Imperial
3
Starting off Week 1
  • Week 1
  • Engineering/Biology Introduction Lectures
  • Journal Club
  • Wiki
  • Brainstorming
  • 3 ideas

4
(No Transcript)
5
Only three ideas made the cut.
6
Bio-Clock (Re-defining Time)
  • Pulse of AHL moves along a gutter of medium
  • Cells fluoresce when activated
  • Refractory period
  • The time period is controlled by
  • the radius
  • gutter width
  • cell density

7
Bio-Memory
10101000000000111110000101010111111000000001111111
11000001010100010100001000010100101001010000001001
010101001010101001101010101001010
  • Data stored in bacteria written using a green
    laser
  • Data read using a UV laser fluorimeter
  • Cells either 1 (RFP) or 0 (no RFP)
  • Data stored in switch

Very High Compression due to small size of
bacteria
8
The Oscillator
  • Culture Wide oscillations of AHL
  • Frequency must be tuned easily
  • Oscillations must be stable

Changing Concentration of AHL
9
  • Week 2
  • Investigation of all 3 ideas
  • Modelling
  • Evaluating risks
  • Start work in the Wet lab
  • Decision on the Oscillator as main projectcan
    use other ideas as further developments
  • Week 3
  • Further research
  • Modelling
  • Assembly of parts
  • Protocols for testing parts
  • Setting up OWW

10
Predator Prey Dynamics
Simply Make a Bio-chemical system that can do
this.
Lotka-Voltarra Model output
11
Design
  • Positive Feedback of A
  • AB Induces production of more B
  • Both A and B are used to make AB

12
Two Cell System
  • Two independent populations of Cells
  • These cells do not kill each other
  • Altering the initial ratios of these cells will
    alter the frequency of oscillations

13
Design Cell1 (Prey)
  • Prey cell must produce molecule A exponentially

Lux R is produced which detects molecule A
Pc
Lux R
Then initiates transcription at Plux
Pc is always on
Lux I
Plux
Which Produces More A
14
Design (isolated models)
Modelling of Prey Cell (system 1)
15
Design (isolated models)
Modelling of Prey Cell (system 1)
16
Design
The Predator Cell
The role of a predator is to reduce the prey
numbers as a function of the predator population
numbers.
Predator
Detect Prey Population Size
Reduce Prey Population Size
17
Design
The Predator Cell
Detects Prey Population Size
Reduces Prey Population Size
18
Design
The Predator Cell
A
LuxR
A
A
LuxR
aiiA
Lux R
aiiA
Plux
19
Design (Entire System)
Diffusion
Extra cellular pool of A (HSL) (this should
oscillate)
The Prey Cell
The Predator Cell
LuxR
A
A
LuxR
aiiA
Lux R
aiiA
Plux
20
Modelling
  • Toms Monster

21
Modelling
22
Outline
  • Further testing of parts
  • Parts assembly
  • ..

23
Thank you
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