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Title: 5 Author: kayxiong Last modified by: kayxiong Created Date: 8/29/2004 1:27:56 PM Document presentation format
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GEL ELECTROPHORESIS Gel electrophoresis uses a ... cellulose acetate paper electrophoresis Slide 16 Gel Electrophoresis AGAROSE GEL POLYACRYLAMIDE GEL DNA ...
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They all cause horizontal streaks. Underfocusing: insufficient focusing Vh. ... (pI change and start moving again.streak) Basic narrow gradients are sensitive to ...
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The proteins that are to be used: Ovalbumin. Casein. Gluten. Theoretical Background ... Gluten = 7.64. Blank result in our film. Possible problems. Solutions ...
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Some Commonly Used Gel Filtration Materials. Purification of Rat Liver Glucokinase. SDS-PAGE sample results. Isoelectric Points of Several Common Proteins. 2D ...
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Zeta potential at break between Stern and diffuse layers ... 2.3 RT F-1 (pCpzc- pC) Proton Surface Charge. pHpzc. Zeta Potential. Isoelectric Point ...
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Casein is the protein found in all mammals’ milk which include cow, sheep, goat, yak, buffalo, camel and human. Casein also exists in pure form by acidifying milk to the isoelectric point at pH 4.6 by either lactic acid or mineral acid.
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Molecular Weight. Amino Acid Frequency. Isoelectric Point. UV ... Molecular Weight & Proteomics. 2-D Gel QTOF Mass Spectrometry. Amino Acid Frequency ...
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isoelectric point, hydrophobicity) Secondary Structure ... Hydrophobicity is a property of groups of amino acids - best examined as a graph ...
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alanine (R = CH3) Ala A. phenylalanine (R = CH2C6H5) Phe F. tyrosine (R = CH2C6H4OH) Tyr Y ... Each amino acid has an isoelectric point, (pI) numerically equal ...
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Electrophoresis. Separate based on size and shape. Fluorescence ... 2D Gel-Electrophoresis. Protein separation. Molecular weight (Mw) Isoelectric point (pI) ...
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Protein sequences access gene sequences and are key to molecular biology ... separates based on molecular weight and/or isoelectric point. 10 fmol - 10 pmol ...
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Isoelectric point (pI) Staining. Birds-eye view of protein abundance. 2D Gel-Electrophoresis ... mass spectrometry doesn't discriminate against novel peptides...
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20 natural a-amino carboxylic acids. 19 are primary amines, proline ... When pH is equal to the pI (isoelectric point) amino acids exist in the zwitterion form. ...
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Lisa G. Smith, R.N., MSN/ED NUR 2242 * Clinical: occurs in MI, CAD, Cardiomyopathy HR not measurable Pt. will be unconscious at this point Absence of pulse, apnea, pt ...
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Clinical and biophysical background (Why) Delineation as a signal processing (How) ... Heart beats are almost identical (requires QRS detection, fiducial point) ...
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Charge Ion Exchange Chromatography Native gel electrophoresis Isoelectric focusing Charges on proteins Different proteins have different native charges.
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PROTEINS. Food Applications. Proteins - Functions. Emulsification Foam Stabilization ... Heat labile. Native form stable at isolectric point (pH 4.6) ...
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Many reasons why there are no corresponding pulse to go with an electrical beat. ... AV node still refractory. pointed! Atrial Fibrillation (a-fib) ...
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He contributed to the advance of electrocardiography and the augmented ... Electrical heart vector is a summary of all cell s vectors in one time point ...
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Lane 1= MW Std, 2= Extraction Method 1, Light Tissue, 3= Extraction Method 1, ... Lane: 1= MW Std, 2= sample before depletion, 3= after depletion. Arrow points ...
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Gel Electrophoresis Steps Breakdown the protein into amino acids Break amide/peptide bond Called hydrolysis (opposite of condensation) Acid or base and heat required ...
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Title: BC basic - from big bang Author: Rong-Huay Juang Last modified by: juang Created Date: 8/15/1997 8:46:50 PM Document presentation format:
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Proteins, Amino Acids, and pH. Weak Base. Weak Acid. Amino acid as a zwitterion. If tyrosine is buried deep inside a protein, it is not readily accessible and a ...
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Title: PowerPoint Author: Handsome Yu Last modified by: Created Date: 10/26/2001 12:27:17 AM Document presentation format:
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General method for synthesis of azo dyes Add a cold aqueous solution of sodium nitrite slowly (with cooling and stirring) to a cold solution of the amine compound in ...
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Zwitterion ... dipolar ion / zwitterion. The dipolar nature of amino acids give to. their ... zwitterion is electrically neutral and exists at specific pH. ...
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II- Classification according to polarity of side chain (R): A- Polar amino acids: in which R contains polar hydrophilic group so can forms hydrogen bond with H2O.
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Amino Acids (Chapter 37B) General Structures. Two contrasting groups -COOH acidic ... Less acidic and basic than normal RCOOH and RNH2. COO- C H3N. H. R ...
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Physical adsorption - van der Waals forces. Electrostatic adsorption ... Cation and Anion Adsorption. Cations. Anions. 0. 100. pH % adsorbed. Sorption edge ...
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Polyprotic Acid-Base Equilibria Introduction 1.) Polyprotic systems Acid or bases that can donate or accept more than one proton Proteins are a common example of a ...
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Chapter 09 Polyprotic Acid-Base Equilibria Proteins perform biological functions such as structural support, catalysis of chemical reactions, immune response to ...
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Polyprotic Acid-Base Equilibria Introduction 1.) Polyprotic systems Acid or bases that can donate or accept more than one proton Proteins are a common example of a ...
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Number of heart beats per minute. Normal value is 60-90 bpm at rest. Stroke Volume (SV) ... of blood pumped by ventricle per minute. CO = HR x SV. Normal value ...
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Centrifuge the sample to separate the insoluble portion (pellet) from the soluble (supernatant) Remove a portion of the supernatant and mix with 'gel' loading buffer ...
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Molecular mass determination by gel filtration chromatography ... Logarithmic relationship between the molecular mass of a protein and its ...
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Ischemia to Infarct. Infarction is an evolving process. As the infarct evolves ECG changes may occur quickly or more gradually ...
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Amines Ammonia derivatives
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This is a method for the separation and identification of proteins in a sample ... http://www.aber.ac.uk/parasitology/Proteome /Tut_2D.html#Section 3 ...
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Introduction: Protein Aulanni am Laboratorium Biokimia Jurusan Kimia FMIPA Universitas Brawijaya
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biochemical methods used in proten purification and characterization biochemical methods used in proten purification and characterization classical methods for ...
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Chapter 14 Proteins
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Possible transfer of vanomycin resistance genes to more serious pathogens is a major concern. ... Peptides are separated by hydrophobicity on reverse phase column ...
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CHE 242 Unit VIII The Structure, Properties, Reactions and Mechanisms of Carboxylic Acids and Their Derivatives CHAPTER TWENTY-FOUR Terrence P. Sherlock
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To attend alternate exam, you must email me with 1) your conflict, 2) which exam ... C) Remains stationary. D) Accelerates to the left. E) None of the above ...
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Amino acids are zwitterion molecule with both positive and negative charge ... Neutral zwitterion. Only ions are H2A , A-, H and OH ...
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ELECTROPHORETIC METHODS Commonly used protein stains An ethidium-stained gel photographed under UV light Western blott technique Commonly used protein stains An ...
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In proteins only L- configuration is found ... Zwitterion: 'hybrid ion' can act as acid (proton donor) or base (proton acceptor) ...
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Naturally occurring amino acids has an amino group (NH2) to the carboxyl group (COOH) ... Since it exists as internal salt, known as zwitterion, ...
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An anion-exchange resin has positive charge and binds negatively charged ... Anions of SDS bind to peptide chain and protein is negatively charged, moves to anode. ...
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Protein Purification and Characterization Techniques Nafith Abu Tarboush, DDS, MSc, PhD natarboush@ju.edu.jo www.facebook.com/natarboush Extracting Pure ...
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Overview of 2DE Complex mixture of proteins Denature and solubilize in solution (Sample prep) Separate proteins by charge in first dimension (IEF)
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Title: OC 27 Amino Acids Author: Bill Brown Last modified by: Bill Brown Created Date: 8/25/1997 6:29:55 AM Document presentation format: US Letter Paper
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amino acids and proteins qca
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