Assessing and mitigating biosafety risks in synthetic biology J. Christopher Anderson UC Berkeley Bioengineering SynBERC Synthetic auxotrophs for containment
A mixture of bacterial cells containing prototrophs, leu- auxotrophs and Lac ... experiment an Hfr E. coli prototrophic strain transfers three of its genes ...
Mutation is created to fit a specific environment condition. Luria & Delbr ck Experiment ... Auxotrophic mutants (v.s. prototrophic wild type) Examples: ...
The speed and magnitude of this genetic change is profound in many ... Remember Beadle & Tatum? Auxotrophic arg- mutants? Procedure requires: Replica plating ...
Start with Salmonella that are his-, auxotrophs unable to make their own histidine. ... Test isn't perfect: Salmonella are prokaryotes, and we have complex ...
Prototrophic: microbes that live on a minimal growth medium inorganic salts energy source. ... Prototrophic: Only dividing cells in minimal media. Killed by ...
http://saturn.roswellpark.org/huberman/BIR572/YeastGeneticsLectureNotes.htm l ... Meiosis I. Meiosis II. from 'Getting Started with Yeast' by Fred Sherman ...
... Protein Synthesis * * Figure 15.3 Method used to determine the ... biochemical pathway leads to ... PowerPoint Presentation Author: myself i Last ...
One of the pillars of genetic analysis. Where have we talked about complementation before? ... They are in the same gene and in the same complementation group. 4 ...
Physiological status of the explant callus induction from the ... by physiological status, e.g. nutrient status, hormonal content, ... lag/conditioning ...
Rice paper cont. TPA = Tissue plasminogen activator, dissolves clots Problem: Cleared quickly from bloodstream by liver Bind to hepatocytes in liver via TPA s ...
Title: A cyanobacteria bloom Author: Dr. Michael Parsons Last modified by: Dr. Jason P Turner Created Date: 1/23/2000 1:11:51 AM Document presentation format
BioWire (Signal Transfer) Paula, Patrick, Jenny, Kang-Xing ... The J. Craig Venter Institute. National Science Advisory Board for Biosecurity (NSABB) Jul'05 ...
Growth & Culture of Bacteria Binary Division 1 to 2 to 4 to 8 to ? Phases of Growth Lag Adapt to nutrients Log Active growth Stationary Death = Growth rate Death ...
... Variable region * OUTLINE OF NEXT LECTURE TOPICS Expression and manipulation of transgenes in the laboratory In vitro mutagenesis to isolate variants of your ...
E. coli as a host PROs: Easy, flexible, high tech, fast, cheap; . . . . . . but problems CONs Folding (can misfold) Sorting - can form inclusion bodies
Wang et al.: double K.O. of splicing factor gene; strategy, coverage of viability ... in cultured lines: myc double K.O. = viable, sick (Skip?) 23. Or (50:50) ...
Microbial Genetics Mutation Genetic Recombination Model organism E. coli Molecular Cloning Bioinformatics Golden Rice Genetic Word List 4 Transduction ...
AICAR = 5-aminoimidazole-4-carboxamide. ribonucleotide. TPP ... Hypothesis 1: A derivative of AICAR serves as signal for infection thread development ...
A semi-quantitative high-throughput screening method. for microbial L -tyrosine ... number of samples to identify putative microbes with altered L- tyrosine production ...
Genetics as a subject (genetic things that go on in somatic cells: ... Recessive mutations (most knock outs) are masked. 10/19/09. 4. Solutions to diploidy problem: ...
CATABOLISM, degradative phase of metabolism accompanied by release of free energy ... THREE STAGES CATABOLISM. DEGRADATION OF LARGE BIOMOLECULES INTO BUILDING ...
... Reliance on pedigrees Possible to determine sex linkage, ... Mutation rate depends on species and on gene Generally, mutations are random Hot spot: ...
Transcriptional Induction of Genes Encoding ER Resident Proteins Requires a ... Functional Characterization of IRE1 in yeast using Viability Assays-Fig. 5 ...
bacteria (where first discovered) to man where they are involved. in a variety of disease ... to reactions from freckling and skin ulceration to skin cancer ...
AICAR = 5-aminoimidazole-4-carboxamide. ribonucleotide. TPP ... Hypothesis 1: A derivative of AICAR serves as signal for infection thread development ...
lysogenic or temperate phage. Transduction. Bacteriophage or ... Generalized transduction lytic or lysogenic phage. any suitably sized piece of DNA packaged ...
Expression in yeast use of Pichia pastoris Alcohol oxidase (AOX1) promoter: Induced by methanol Allows for high levels of foreign protein expression ...
Genes for biosynthetic enzymes can be amplified using multicopy plasmids ... citric acid output. Eukaryotes: compartmentation. Yield Optimization. in Aspergillus ...
... A codon for one amino acid is replaced by another Synonymous substitution: ... Loss of function mutations: Gain of function mutations: Less common than loss Why?