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Dorzolamide. A topical Carbonic anhydrase inhibitor. Ampholytic characteristics, ... scanning laser ophthalmoscope angiograms (SLO) ...
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Describe the R groups of amino acids in terms of polar, nonpolar, aromatic, ... Substances having this dual nature are amphoteric and are often called ampholytes. ...
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Soluble ampholyte based gradient dynamic and transient. Immobiline based gradient fixed, allows focusing to proceed until equilibrium ...
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Title: PowerPoint Author: Handsome Yu Last modified by: Created Date: 10/26/2001 12:27:17 AM Document presentation format:
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They all cause horizontal streaks. Underfocusing: insufficient focusing Vh. ... (pI change and start moving again.streak) Basic narrow gradients are sensitive to ...
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Overview of 2DE Complex mixture of proteins Denature and solubilize in solution (Sample prep) Separate proteins by charge in first dimension (IEF)
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Aminos uren Von: Imke Sp . und Jana Inhalt Allgemein Funktionen Chemischer Aufbau Einteilung der Gruppen S ure-Base-Eigenschaften Essentielle Aminos uren ...
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Title: M todos de Isolamento de Biomol culas Author: Celia Carlini Last modified by: usuario Created Date: 3/25/2000 1:05:34 AM Document presentation format
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Biochemistry
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Title: BC basic - from big bang Author: Rong-Huay Juang Last modified by: juang Created Date: 8/15/1997 8:46:50 PM Document presentation format:
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ELECTROPHORESIS Definitions Theory of Electrophoresis Electrophoretic Technique General Procedures Types of Electrophoresis Technical Considerations
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Bacterial Physiology A Proteomic Approach to Oral Diseases Peter Zilm Microbiology Laboratory Dental School The University of Adelaide
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Universidade Federal de Pelotas Centro de Desenvolvimento Tecnol gico Proteoma Baseado em Eletroforese 2D: Fundamentos e Aplica es Dr. Luciano da Silva Pinto
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Reactions in Aqueous Solutions I
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2nd dimension: SDS-PAGE. Gel staining (SYPRO ruby) Imaging (Fuji imager - 470nm) ... long term storage at 80 C. Limit sample contamination. gloves (keratin) ...
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Disinfection Disinfection Disinfection The removal of harmful organisms (but not usually spores) or the reduction of numbers of organisms to a level where they ...
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Molecular mass determination by gel filtration chromatography ... Logarithmic relationship between the molecular mass of a protein and its ...
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... a priori classified as needing curation (series B) were topped up with alcohol. ... Standard topping up procedure hardly improves the pH. Statement of problem: ...
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GEL ELECTROPHORESIS Gel electrophoresis uses a ... cellulose acetate paper electrophoresis Slide 16 Gel Electrophoresis AGAROSE GEL POLYACRYLAMIDE GEL DNA ...
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Chapter 4 Amino Acids Read entire chapter Learn to draw the 20 standard amino acids. Learn both the 3 letter and the 1 letter codes. (pages 66 & 67)
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Introduction: Protein Aulanni am Laboratorium Biokimia Jurusan Kimia FMIPA Universitas Brawijaya
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The buffer's pH is a function of it's pKa and the ratio of concentrations of ... Preparation of A Buffer. To prepare a buffer first choose an acid with a pKa ...
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Within this range there exists a ratio between the charged and uncharged groups ... zwitterion. Different pKa. Effects of Ph on Amino Acid R-group Charge. pK=pI ...
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other AAs are formed by a posttranslational modification ... amphoteric electrolytes) 'AMPHION' Important reactions of AAs. dissociation (formation of salts) ...
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... not bleach or color stable. Benzene groups are acid, alkali and bleach stable ... Not compatible with hypochlorite bleach. Hydrolyzes under acidic conditions ...
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Gel Electrophoresis Slab for proteins and DNA Cooling/sieving mechanism polyacrylamide Capillary isotachophoresis Capillary electrochromatography Micellar ...
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Chimie Terminale S Enseignement de sp cialit
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Introduction to Hydrogeology (GEO 346C) Lecture 6a: Hydrogeochemistry Instructor: Bayani Cardenas TA: Travis Swanson and John Nowinski www.geo.utexas.edu/courses/geo346c/
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It provides review in acid base balance and imbalance of biochemistry in nursing science
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Concentration - Ultrafiltration. Precipitation. Preparation of proteins, cont ... Typical solubility behavior of protein as a function of pH and salt concentration ...
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Methodology for the Commassie staining Proteins that are separated by the 2-DE has to be stained by the staining solution for visualization. Many staining methods are ...
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large pore size of gel and equilibrium conditions minimize molecular sieving ... mixture of aliphatic amines either carboxylic or sulfonic acid groups ...
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Relatively few medical breakthroughs derived from ... Bacillus subtilis. Bifidobacterium longum. Borrelia burgdorferi. Brucella suis. Buchnera aphidicola ...
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Only 'Proteomics' is the large-scale screening of the proteins of a cell, ... Using abrasive vortexed bead to break cell walls. 2. Cell disruption (continued) ...
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Chapter 09 Polyprotic Acid-Base Equilibria Proteins perform biological functions such as structural support, catalysis of chemical reactions, immune response to ...
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Protein chemistry to proteomics Technological advancements in protein analysis with increased sensitivity, resolution and capability to carry out high throughput ...
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Nanofluidic Microsystems for Advanced Biosample Preparation
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Devices for measurement of Osmolar concentration (measurement is based on cryoscopy), Diffusion Viscosity (practical exercises) ... mentioned in lecture about MRI) ...
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Lehninger Principles of Biochemistry, Fourth Edition, 2005 Chapter 3 AMINO ACIDS, PEPTIDES, AND PROTEINS
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Chap. 3B Amino Acids, Peptides, and Proteins Topics Amino acids Peptides and proteins Working with proteins The structure of proteins: primary structure
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For all the standard amino acids except. glycine,the a-carbon is covalently bonded ... Steric relationship of the stereoisomers of alanine to the absolute ...
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Mode of Action of Non-Antibiotic Antibacterial Agents. ... Bacteriostasis/Fungistasis. Inactivation of Preservatives by Dilution. Filtration ...
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Title: Balancing and using redox equations Author: xyz Last modified by: qw Created Date: 5/13/2003 3:37:11 AM Document presentation format: On-screen Show
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Acid - Base properties of amino acids ... Diastereomers are not mirror images and have different chemical properties. Stereoisomers ...
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Wound dressing POLYMERIC IMPLANTS Biodegradable suture Intraocular Lens Contact Lens * * Polymers: Biomedical Applications Rubbers latex, silicone good ...
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Protein world: study of less abundant proteins ... Passive elution of proteins. Analyze in a linear MALDI-TOF MS. Peptide mass FINGERPRINT: ...
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erg = dyne . cm. Its equivalent to the surface tension ? ... The force of detachment is recorded in dynes. on a calibrated dial ...
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Application of Proteomics in Biological Research An introduction Jau-Song Yu Department of Cell and Molecular Biology Chang Gung University (?????) MALDI-TOF MS ...
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957 putative interactions. 1004 of 6000 predicted proteins ... 1200 putative interactions identified. Connects 45% of the H. pylori proteome. Lecture 1.1 ...
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BEAMing: PCR on beads compartmentalized in a wateroil emulsion. Second view.
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PROTEOMICS OF OBESITY
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Basic Building Blocks: Proteins Largest variety of biomolecules Most of the weight of cells, aside from water Basic unit is amino acid Form of amino acid
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