DNA damage and repair - PowerPoint PPT Presentation

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DNA damage and repair

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Title: DNA damage and repair


1
  • ??????-?????????? ?????? ??????????? ?
    ???????????? 6. ???????????????? ??????????????
    ???????.
  • ?????????, ??????????? ??? ???????. ???????????
    ?????????? ? ???????????? ??????. ???????
    ????????????? ??????????. ?????? ??? ???????????

2
?????????? ?????? ???????????????? ???????
??????? ? ????? ???????????
  • 1. ???????????????? ?????????? ?????????? ?
    ???????????? ???????? ?????????

3
?????????? ?????? ???????????????? ???????
??????? ? ????? ???????????
?????????????? ??????????????? ???????
???????
S1gtgtS2
?????? ???? ???????? ??????? ?????????????
??????????
4
?????????? ?????? ???????????????? ???????
??????? ? ????? ???????????
??? ????? ? ???????????????? ???????????
?????????? ????????? ???????? ???????
vk3ES1S2 ??? ?????????? ES1S2 ? ???????
????????? ??????????? ????????? ?????????????
??????? ? ??????? ??????????????????? E0 E
ES1 ES1S2
5
?????????? ?????? ???????????????? ???????
??????? ? ????? ???????????
DE k-1k-2k2S2k3k-1k3
DES1
k1S1k3k1S1k-2 DES1S2 k1S1k2S2
6
??? ??????????? ?????????? ??????? ??
????????????????? ?????? ??? ????????? ?????
?????????????

7
??????????? ??????? ????????????? fi
  • ?????????? ???????? ??????? ?? ??????? ???
    ????????? ????????????? S1 ? S2const, ???
    ????????, ??? ????????? ?????????????
  • S2 ? S1const.

8
??????????? ??????? ????????????? fi
  • af0f2/S2 bf1f12/S2

9
?????????? ?????? ???????????????? ???????
??????? ? ????? ???????????
  • 2. ???????????? ??????? ????????????? ????
    ?????????? ? ???????? ??????????? ????????
    ?????????

v ??? DES1S2 ???????????? ???? ?????, ?
??????? ?????????? ??????????? ?????????
???????.
10
??????? ????????????? ??????????
?? ??????????? ??????? ?????????????
?????????? ???????, ????? ?????? ??????? ?????
??????????? ??????????? ???????? ? ??? ?????
?????????????? ????????? ???????.
11
?????? ??????????? ??????? ?????????????
??????????
  • ????-??????????.
  • ????? ???????? ? ???? (gel retardation, ???
    electrophoretic mobility shift assay EMSA).
  • ??????? ?????????????. ????? ??????? ??
    ?????????? ????????????? ????????????? ????????
    ?????????? (Trp) ? ???????? ???????? ????????,
    ???????????? ??? ????????????????????.
    ????????????? ????????????? ??? ???????????
    ????????? ? ????????? ????? ?????????? ? ???
    ?????????. ? ???? ?????? ????? ????????????
    ?????? ?????????, ??????? ?????????????? ?????.

12
????????????? ???????? ?????????? ??? ???????????
??????? ????????????? ??????????
  • ??????? ?????????? ?????????? ????? ???????,
    ????? ??? ?????????? ???? ???????????, ?? ????
    ??????????? ? ?????????????? ???????
    ??????????????? ????????.
  • ? ?????? ??????? ????????? ????????????
    ?????????????? ?????????????? ??????? (KM, kcat)
    ????? ?????????? ??? ??? ??????, ? ???????
    ????????? ??????? ?????? ???????? (??? ???????).

13
??????????? ??????? ????????????? ??????????
??? ???????????? ??????? ????????????? ??????????
? ??????? ?????????????????, ??????????????
?????????-????-???????????, ????
???????????? ????????????? ??????? ???????????,
????????
????????-???????????
???????????
14
????? ???????????? ???????????
  • ??????? ????????????????? ???? ? ????????????
    ???????????-????, ??????????????
    ???????????-????-??????????)

E ??? Phe ??Phe??? ??i ??Phe???
????Phe ? Phe????Phe ???
15
????? ???????????? ???????????
  • ??????????? ??? ?? ?????? ???????? ????????? ?
    ???????? ????? ??????
  • ????????? ?????? ????????? ? ????????? ?????????.
  • ????????????? (Phe-ol) ???????????? ?????????
    ???????????? ? ??????? ?????????????????.



Kd (Phe)10-6M Kd
(Phe-ol)10-5M ???????????
?????????????
16
????? ???????????? ???????????
  • ????????? ????????
  • ???????? ? ?????????

2. ????????? ???????? ??????? ???????? ES1I
??? ???????????? ??????? ????????????? ??????????
????? ?????? ????????? ??? ???? 1 ? 2, ? ???????
?????? ??????.
17
  • ???????? ???????????????? ???? ?????, ? ?.?. ?
    ??????? ?????????????????, ?????? ??????????
    ???????? ?????????????????? ????????
    (?????????-???), ???? ???????? ???????? ?
    ??????????? ES1 ????? ???? ????? ???????, ???
    ???????? ???????? ?? ?????? ?????????? ?S2.
  • ??? ????????????????? ????Val ?? ?????? ?????
    ?????????? ????????? ???????????? ???
  • ? ??? Val ????Val ??i
  • ??????? ? ???????? ?????? ????????? ?? ????????
    ??? ??????????? ??? ????????? ??????, ???????
    ???????? ??????????? (??i), ? ?? ?? ?????
    ?????????? ????????????. ???????????? ??????????
    (?????????????????) ??????? ?? ??????????
    ????????? ???????????????????? ??????
    ??????????????? ??????? ? ???. ???????? ????????
    ? Val ??????? (Kd10-6M), ??? ???????? ? ???
    (Kd10-3-10-4M), ?? ??????? ??? ?????
    ?????????????????? ?????????? ?????????? ???????
    ?? ????????????.
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