NK Cell

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NK CellWhat I Know

• ?????
• ???

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NK CellWhat I Know
NK?????
NK?????????????
NK???????
NK???????
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NK?????

• NK????????????,?????"natural killer
  cell",???"??????",???????????????????????,????????
  ???????,?????????????????"????????????????????"?

??
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NK???????
a. NK??????,????????????,?????????????
b. NK??????????FasL?????,???????IFN-?????????


c. NK????????T ? B????????
d. ???? IL-2????IL-2????
e. NK???????????????????????????
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NK???????
??ADCC,????????????????
???????????????,???????????????????????
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NK???????

• NK???????????????????,???NK???????????
• a.  NK?????????????????????
• b. NK????IFNr???

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NK???????

• NK???????(ITIM)
• NK ??????? (ITAM)
• ??

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1.1???????????
1.2 C?????????
1.3 KIR?MHC?????
1.4 ?????????
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NK???????(KAR),??????????????????,1990?Karre??????(missing self)??,?? NK???????????MHC-1??????,?????????????,??????????MHC-1????????,KIR??????????????,??NK???????????,??????????
2.1?MHC?????KAR?
2.2 MHC?????????
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??
???????KIR?KAR????,????????????????,?????????NK????,??MICA/B????NKG2D?????????NK?????????????,?????MHC ?MICA/B????????,??MHC?????????????????
??
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????
?NK???????????????? NK???????TGF-ß??,?????????????? ????????NK???????????,????????,??????????????????????????????????????,???????????????????Ig A ?????????,????,NK???????TGF-ß???????????????????????????,????NK?????????? TGF-ß?TGF-ß????????-????????????? ????NK?????????????????????????????????????????,???????????????????????????? TGF-ß??????????????????,???????????????????,?????????????????????
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??NK????????
????????????????????NK??????????????NK???????????????,???????????????IL-2????,??LAK????????NK?????????????????????????????
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    ?1975??????NK????,??????????????????????????????,???????????,???????NK????????????????????,??????????????????????NK?????,??????,??????NK????????????????????????,??????????? ???,????????????YAC???????,4???,???????????????????????,?????NK???????????????70?????NK??????????????
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???,????B16??????????,??????,???????????????100?????40?,??????????????NK??????????20??,???????????,??????????,?????10,000?????,?????????????????????????,??NK???????,????100????,20???????????????????NK????????????????     ???,????B16??????????????,2?????????????????????,?????NK?????????????????60?????NK???????????????????????
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?. ??????????????????

??NK??????????????,???NK?????????????????????NK?????????,?????????????????????? ??????????????????,???????????????,???NK????????????????????????????NK?????,?????????????????? ????????NK?????????????????????????????????????????????????
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CONCLUTION

• ????????NK?????????????????,?????????????,????????
  ?????????,???????????,???????????????????NK???????
  ???,????????????????????,???????????????????

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????
1?????? ????????? ??????? ?? 2????????????????? ??? ?? 3? ????????? 2003??4? 191?---194? 4? ??????? 2003??3?
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The End
The End
Thank you for your listening!!
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1.1 ??????????? ??????NK???????????MHC-I????MHC-I
???????????????????????HLA-A.B?C??????????,???????
?HLA-I???HCA-G.??NK???KIR???I????????????????,????
????,???,???,?????2??3?Ig ????,??NK??
?????????????????????????????(ITIM)??????,?KIR????
??,ITIM????????,SHP-1?SHP-2 ???????,?? NK???????
??
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1.3 KIR?HLA????? KIR2D??????????Ig????????HLA
????? a1 ?a2 ????KIRs?HLA-I???????????,?????HLA??
??????? KIR2DL1 ??? a???77???80???????????
???????????? KIR2DL2 ?KIR2DL3??????,?77???80??????
?????????HLA-C???????KIR2DL4 ? HLA-G??????,???KIR?
???,?????????????,?????????????????(ITIM)?
??????????????????,?KIR2DL4?????????????????,???NK
??????????????????????????NK??,KIR2DL4??????????,?
?NK??????IFN-???????KIR2DL4??????????,??
NK?????????,???NK????????????????????????,??KIR2DL
4 ?????????????? ???KIR? HLA ???????????,?????????
????,????????,????????KIR?????????  
??
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1.2 C????????? CD94/NKG2???NK???????????,CD94/NKG
2A ?????MHC-1???HLA-E??????????,CD94
??????NK2A?B?C? E ??????,?????NKG2A????????ITIM,CD
94??????,??CD94/NKG2A????????????????HLA-E???NK???
?CD94/NKG2A??,???????NK????????
??
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1.4 ????????? ???2??4????C2??????????
???????????????,??ILT-2?3?4?5?LIR-8???ITIM?????,??
????????ILT-1?7?8?LIR6?????? ITIM
??,???????????????????,?????????ITAM
???FCR???????????
??
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2.1 MHC?????KAR?
NKp46?NKp44?NKp30???I g??????,???CD3??FCeRI
???DAP-12???ITAM??????????,??????NK????????NKp46??
?????NK?????,??????????????,?????????????????????,
?????????????????????NK???????,???????????????????
???,????MHC ??????????????,???????????????? ??NKp4
4,????????????,???????V????,???????????ITAM?????DA
P-12??????????,NKp44????????IL-2???NK???,???NKp46?
??????????HLA ???FC?R????????NKp30??????????V
????,?????NKp46,???????NK???????,??????NKp30????NK
p44?NKp46????????????
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NK????IFN r(???)
???????????? NK ???? r ????IFN r??????????????????
????,??????T????????NK???????????????,??IFN
r???????????????????????? IFNa?IFNß?????????,??IFN
r?????T??????????????????
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 NK?????????????????????
?????????,??????????NK???????????,????????????????
????????????????NK??????????????,NK???KIR?NK??????
?????,?????????,??????? HLA?A?B?C??????MHC-1??????
????????????????MHC?????,??,????KAR?????NK
???????????????????,??NK????????????,????,????????
????????,???????????????????????? ??????????T?????
?????,???????????(???,??)????????????????????C9???
??,????????????????,????????NK
??,?????T??,???????????????????????FASL??,????????
? NK????IL-2????????????(LAK)??,LAK???????????????
?
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2.2 MHC????????? MHC?????????KIR2Ds?KIR3DS?KIR2DL
?KIR3DL?????NK???,???????,???39?AA??,??ITIM??,????
?????????????,?????KAR ??????,?????????
ITAM???????