Plasmid (BAC)

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Lots of tools for cloning
Plasmid (BAC) Phagemid Cosmid Lambda (PAC)
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Needs to be at least 40kb to grow, but A left
and right arm can be made smaller See next slide
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MNGTEGPNFYVPFSNKTGVVRSPFEAPQYYLAEPWQFSMLAAYMFLLIML
GFPINFLTLYVTVQHKKLRTPLNYILLNLAVADLFMVFGGFTTTLYTSL
H GYFVFGPTGCNLEGFFATLGGEIALWSLVVLAIERYVVVCKPMSNFRF
GE NHAIMGVAFTWVMALACAAPPLVGWSRYIPEGMQCSCGIDYYTPHEE
TNN ESFVIYMFVVHFIIPLIVIFFCYGQLVFTVKEAAAQQQESATTQKA
EKEV TRMVIIMVIAFLICWLPYAGVAFYIFTHQGSDFGPIFMTIPAFFA
KTSAV YNPVIYIMMNKQFRNCMVTTLCCGKNPLGDDEASTTVSKTETSQ
VAPA
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Logo for a) Splice acceptor
b) Splice Donor
c) Initiator Met
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