Research Template

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G. Brn3a
Methods Animals Mice C57BLk6. All animals were
cared for according to institutional guidelines
prescribed by the UMKC IACUC Committee, the UMKC
division of laboratory animal medicine, and the
National Eye Institute. Adult mice 8 to 12 weeks
of age, were anesthetized by CO2
asphyxiation. Western blots Mouse retina and
brain tissues were lysed in HEPES buffered saline
plus a protease inhibitor cocktail
(Thermo/Pierce) and 2 mM EDTA. Western blots were
probed with anti-VNUT, and ?-actin as a control
housekeeping protein. Immunohistochemistry
Immunostaining was performed using the indirect
immunofluorescence method. Confocal Imaging All
images were acquired sequentially to avoid
spectral bleed through on an upright fixed stage
confocal microscope (Nikon C2 confocal
microscope).
H. VNUT
I. Brn3a VNUT