???:????(cholesterol)

1
??? ? ? ? ?
???
2
?.???????????????????? ?????????,????????
?????????????
(triglyceride,TG)
??
??(phospholipid,PL)
??
???????(cholesterol)
??(glycolipides)
3
???????????
? ???????????
? ??????????????
? ???????????,????????
? ????????
????????,??????????
4
?????????
???????
16 0 ???(???) 18 0
??? 18 1?9 ?? 18 2?9.12
??? 18 3?9.12.15 ???
?????
5
??? ?????
?????????
6
??????
7
???
???
8
??? ??
9
????????
(?)????????? (?)?????? (?)??????????? (?)?????????
??
10
Knoop?????
???????????????,?????,????????????,?????????????
??
?????????????????????
??
11
(?)???????ß-??
????????????,a?ß-??????????,ß?????????,???????????
???,???????CoA ?
12
???ß- ??
??????(??)
??CoA?????
????ß-??(???)
??CoA???????????(???)
13
1. ????????CoA
??CoA???
RCOOH
CoASH
RCOSCoA

???
??CoA
Mg2
ATP
AMPPPi
H2O
?????
2Pi
14
2.??CoA?????
????????????????,???????????CoA(12C??)?????????
???,?????(carnitine,L-ß-?-?-??????)???????????????
???
15
????????A?????
???????ß-???????,???????-?????,?????CoA???????
????
16
3. ??CoA?ß-????
??CoA????????,????ß-?????????,????a,ß-?????????
???????????4?????,?????a?ß-??????,??1????CoA??2???
????CoA ?
17
(1) ? ?
(2) ? ?
H2O
18
(3) ???
(4) ? ?
19
???ß????
??CoA
??
??
L()-ß???CoA
???
ß???CoA
??
??CoA??CoA
20
4.???ß-???????
1?????(16C)????????CoA??????7?ß-??,??????
???CoA 7FAD7NAD 7CoA-SH 7H2O
8??CoA 7FADH2 7(NADH H)
1???????????? (27)(37)(128)131??ATP
??????????ATP?2?????????129??ATP?
21
(No Transcript)
22
TCA
23
????????
?????
24?
8?
24
????????
??????????????? ??????????????????
25
(No Transcript)
26
(?)????????ß-??
?????????????????????????????????ß-?????????????,?
???????????3-?,?2-???CoA??????
27
??????????

28
(?)????????ß-??
???????????(182?9,12 )??????????
?3-?,?2-???CoA??? 2,4-???CoA???
29
??????????
30
(?)????a????????a-???????????????,??a-???????????
??,??????,??1??CO2??1????????? (?)??????????????
?(?-?)???????????-???,??????a,?-???,??????ß-???
31
??? ?????????
?????????? ?????????? ???????????? ???????
32
??????????
1????? 2????? 3????? 4??????ß-????
33
1. ????
???????????????????????????????,???????????????
,???????,???????89??
34

2. ??????

• ??????????????CoA,????????

• ?? CoA??????-?????(?????????)?????,???????????

• ATP?NADPH?HCO3-(????)?Mn2??

??NADPH??????????????,???????????
35
???????
36
(No Transcript)
37
??????
3. ????
(1)?????CoA????????
??CoA???
HOOC-CH2COSCoA ADP Pi ?????CoA
CH3COSCoA HCO3- ATP
Mn2????
???
38
(2)?????????
39
E.coli???????(ACP)
?????????????,?77???????????4-????????,???????
,???ACP-SH,???????????????ACP-SH???????????
40
ACP
O-CH2-Ser-ACP
HS
??4-????????
CoA
41
???????????
?
?
?
?
ACP
?
?
???CoAACP??? ??????CoAACP??? ?ß-???-ACP??
?ß-???-ACP??? ?ß-???-ACP??? ????-ACP???
42
(3)??????????
??
???
C2???
??
??
??
43
???(16C)???????
??CoA7?????CoA 14NADPH14HH2O
??????? (7???)
???14NADP 7CO27H2O8CoA-SH
44
(4)?????????? 1.???????????????????,????2??? 2.?
????CO2 3.??????????????? 4.????NADPH,??????2??
NADPH 5.?????ACP?
45
4??????????ß-????(???)
???? ß??
??????? ??? ???
???? ACP-SH COA-SH
???????????? ?????COA ??COA
??????? NADPH FAD?NAD
?? ?????????? ???
?? ??7?ATP?14NADPH ??129?ATP
???????????? ?? ???
?????? ??????? ??????
???? ?????? ??????
???? 7? 7?
46
??????????
? ???CoA???????,???????????????????????????,????
?????????
???????????ß-??????,??NADPH ???????????????
????
????????????????,????A??????,???????A???????
47
???????????

• ?????4,?5,?8??9????,???????????,????????????

• ???????????9???????,???10?C??????????????

• ????? ???????,???????????,?3?

???(18C2?9,12
???(18C3?6,9,12 )
?????(18C4 ?5,8,11,14 )
48
???????
????????????, ???????????
49
1???????
DG
MG
CoASH
TG
50
2????-??????
???
???
TG
51
???

• ???????????????,?????????????,?????????

52
(No Transcript)
53
The end
54
CH3COCoA
CO2
HOOCCH3COCoA
ACP
CH3COCoA
CH3COACP
HOOCCH3COACP
CO2 ACP
C2
C2
C2
C2
C2
C2
CH3COCH2C0-SACP
ß-???ACP
CH3(CH2)14C0-SACP
NADPH
CH3CH2CH2C0-SACP
??ACP
NADP
CH3CH(OH) CH2C0-SACP
ß-???ACP
NAD P
NADPH
CH3CH CH2C0-SACP
H2O
ß-???ACP