????????????,???????????????????????????????????????,??????1012?,?????1014?,??????????????????,????????????????????????????????????(cell cycle)????,???????????????????????????????????????????????,??????????????????

1
??? ???????
????????????,???????????????????????????????
????????,??????1012?,?????1014?,??????????????????
,????????????????????????????????????(cell
cycle)????,???????????????????????????????????????
????????,??????????????????
2
??? ????????? ?????? ?????????????????????
?????????(????????,???????),????????G1?(gap1),???
???????DNA??????????S?(synthesis
phase),?DNA?????,???????H3-TDR????????DNA??G2?(ga
p2),?DNA???????????????????M???D?(mitosis or
division),??????????G0???????????,????,????????,?
?????, G0?????G1?? G0???????,?????????(???????????
??)?
3
????
4
??????4???
5
????????,????????????????????,???????????????
????,?????????????????????????,?????????????????,?
G0???,?????????????????,???????????,???????,??????
,???????????????
6
???????? G1????????????,????DNA,????????????G1??
S????????????(????,????),??????????? S?DNA??,????
?????????,?????? G2????????,????M?,?G2???????? M?
????
7
Experimental demonstration of the coordinated
Synthesis of DNA and hitones.
8
???????? ????????????????,???????????????10??
,???????24??,????18??,?????????18??,CHO??14??,HeLa
??21??????????G1????,??????????????? 1?????DNA????
?????????? H3-TdR??,??,??,????,???????
BrdU??,?????? 2??????????? ??DNA??????,?????????
?????
9
??????????(??????)
TG2-G2???,TM-M???,TS-S???,TC-?? ????,TG1 TC
-(TG2 TM TS )
10
???????????
11
???????
12
????? ?????(synchronization)??????????????????
????????,????????,?????????? 1?????? ????????????
,????????,????????????????????,???????,?????108,??
?69cm? ????????????????????,???3?????????,??????
????,?9?????????????? ???????????????????????????
,??????,?????
13
2?????? ????? ??????????????????????,???????????
??????,?????????,??????,M???????,???????,?????,???
?????,??????,??????????????????,????,??????,??????
??,?????????????(??????12) ??????????????????,
?????????????????????????,????????????????????????
????,???????????
14
(No Transcript)
15
????? DNA???????DNA??????,?????DNA??,????????????
?,??????????S??G/S????5-????????????????????????AD
R?GDR?TDR,????DNA??????????????TDR?S????????,????T
DR?????????????????????,??????????????????????????
???????,????????? ??????????????????????,????????
????????,??????????????????,?????????
16
TdR??????????
17
??????? 1?????????(????????) G1?G2???,???????,???
???
18
2??????? 60-90??,???????
????????
????????
19
3??????? ????,????????????????
20
4,??????? ????,???????? DNA?????(G1)?10??DNA??(S
)?40???????(G2)?20??????70???????????????,??????
35???