E-MAIL: [e-mail]

1
?????

• ???? ? ? ?
• E-MAIL Liujiping_at_scau.edu.cn
• ????????????????
• ??????????
• 2003?2?

2
????

• ?????????????,??????
• ???,????????????,????????????,???????????????,????
  ???????????,??????????????????????????,???????????
  ?????????????????????

3
Bioinformatics ??????????

• ?BIOINFORMATICS ??????, ?????????????,?????RD,?BI
  OINFORMATICS????????????,RD???????????????BIOTECH
  ??????BIOINFORMATICS ???????????,????BIOINFORMATI
  CS?????

4
??????--????

• BIOINFORMATICS CERTIFICATION,???????????????????
  ????????
• Visual Basic      -- 1195
• Visual C                   -- 1295
• BioInformatics -- 2500
• MIT(Massachusettes Institute of Technology)
• (??)?????? Course 20.01s Date June 24 - 28,
  2002 Tuition 2,500

5
??? ????????????

• ???

6
????

• 1???
• 2???
• 3???
• 1)????????
• 2)???????????
• 3)???????
• 4?????

7
?. ?????????

• Genome informatics is a scientific discipline
  that encompasses all aspects of genome
  information acquisition, processing, storage,
  distribution, analysis, and interpretation.
• ????????,???????????????????? ????????????
• (The U.S. Human Genome Project The First
  Five Years FY 1991-1995, by NIH and DOE)
• ??????????????????????????????
• ?????????????30??????100?????

8
??????????

• ??????????,???????
• ????????????????????????????????PC???
• ?????????????????????????????????????(Interface)
  
• ?????????????????????????????????????????????????
  ?????????????????????????????????????,???????????
  ??

9
???????????

• ?????(Bioinformatics)----?????????????????????????
  ???????????????????(??????)???????????????????????
  ???????????????????????????????????????????????,??
  ????????????????,?????????????????????????????????
  ?,????????????????????????,???????????????????????
  ,????????????????????????

10
??????? 18??????DNA???? 14??????
11
???GenBank??DNA?????????,???14????????1999?12?????
?30?,????47000????2000?4?DNA?????60????,2001??????
??110???????EST????600???,?????EST?????300??,????
????90?????1.8Mb???????(Haemophilus
influenzae Rd)??????1995???(Fleischmann et
al.,1995)??,??54????????????????,????9?????31?????
??14????????????????????,?????????????????70??????
?????????
12

• ????????GenBank?1979?????,1982?????
• ???????????EMBL?????1982?????
• ???1984??????????????DDBJ,??1987?????????????,DNA?
  ???????80??????????,???????????110???!????,?????18
  ??,???????????

13
Dbcat????????????? ?? ????? ??
?????DNA 87 RNA 30???
94 ??? 58???? 30 ?????
18?? 43 ?? 153
14

• ???????1.2??????????2000?2????????
• ?????????????,??3300???????22??????1999?11?????,??
  ???1999?12?2??Nature ????22????????679???,??55???
  ??????35????????????,?????????????????????
• ?????????????????,????90???????????????2000?4??
  ??,?2003????????99????????????????3?????
• ???????????????????14015?(?http//www.ncbi.nlm.ni
  h.gov)

15

• How many characters are in the Heaven Book?
• 3109 10,000 books
• 
  1 book 100 pages
• 1 page
  3,000 characters
• CCGGTCTCCCCGCCCGCGCGCGAAGTAAAGGCCCAGCGCAGCCCGCGCT
  CCTGCCCTGGGGCCTCGTCTTTCTCCAGGAAAACGTGGACCGCTCTCCGC
  CGACAGTCTCTTCCACAGACCCCTGTCGCCTTCGCCCCCCGGTCTCTTCC
  GGTTCTGTCTTTTCGCTGGCTCGATACGAACAAGGAAGTCGCCCCCAGCG
  AGCCCCGGCTCCCCCAGGCAGAGGCGGCCCCGGGGGCGGAGTCAACGGCG
  GAGGCACGCCCTCTGTGAAAGGGCGGGGCATGCAAATTCGAAATGAAAGC
  CCGGGAACGCCGAAGAAGCACGGGTGTAAGATTTCCCTTTTCAAAGGCGG
  GAGAATAAGAAATCAGCCCGAGAGTGTAAGGGCGTCAATAGCGCTGTGGA
  CGAGACAGAGGGAATGGGGCAAGGAGCGAGGCTGGGGCTCTCACCGCGAC
  TTGAATGTGGATGAGAGTGGGACGGTGACGGCGGGCGCGAAGGCGAGCGC
  ATCGCTTCTCGGCCTTTTGGCTAAGATCAAGTGTAGTATCTGTTCTTATC
  AGTTTAATATCTGATACGTCCTCTATCCGAGGACAATATATTAAATGGAT
  TGATCAATCCGCTTCAGCCTCCCGAGTAGCTGGGACTACAGACGGTGCCA
  TCACGCCCAGCTCATTGTTGATTCCCGCCCCCTTGGTAGAGACGGGATTC
  CGCTATATTGCCTGGGCTGGTGTCGAACTCATAGAACAAAGGATCCTCCC
  TCCTGGGCCTGGGCGTGGGCTCGCAAAACGCTGGGATTCCCGGATTACAG
  GCGGGCGCACCACACCAGGAGCAAACACTTCCGGTTTTAAAAATTCAGTT
  TGTGATTGGCTGTCATTCAGTATTATGCTAATTAAGCATGCCCGGTTTTA
  AACCTCTTAAAACAACTTTTAAAATTACCTTTCCACCTAAAACGTTAAAA
  TTTGTCAAGTGATAATATTCGACAAGCTGTTATTGCCAAACTATTTTCCT
  ATTTGTTTCCTAATGGCATCGGAACTAGCGAAAGTTTCTCGCCATCAGTT
  AAAAGTTTGCGGCAGATGTAGACCTAGCAGAGGTGTGCGAGGAGGCCGTT
  AAGACTATACTTTCAGGGATCATTTCTATAGTGTGTTACTAGAGAAGTTT
  CTCTGAACGTGTAGAGCACCGAAAACCACGAGGAAGAGAGGTAGCGTTTT
  CATCGGGTTACCTAAGTGCAGTGTCCCCCCTGGCGCGCAATTGGGAACCC
  CACACGCGGTGTAGAAATATATTTTAAGGGCGCG
• 
  (1250 characters)  
• ????????????????????

16
??????????
? ?? ?? ?? ??? ?? ?? ??? ???? ?? ?? ???? ?? ?? ???? HIV ? ???
17

1. ???????????????60???????10???????60?????20???,??3-
   4??,?????
2. ??,?80????,????30??,???????6-7???
3. ?90???,???????40?????10??,??????1???
4. ?2000?,?????50??,???5??,????10??(??http//www.ncbi
   .nlm.nih.gov??PubMed????)?

18
1?????

• ?????????????????????????????????????????????,????
  ??????????????????????,???????????

19
????????????????

• ???????????????????????HGP???????DNA??????????????
  ???????????????????????????,??????????????????????
  ?????????????????????????,????????????????????????
  ?????????????????????,??????????????????,?????????
  ???????????????????,???????????????????????

20
2???????

• ??,??????????????,??????????????????????????,?????
  ?????????(National Center for Biotechnology
  Informatics,NCBI)??????????? (European
  Bioinformatic Institute,EBI)??????????(Center for
  Information Biology,CIB)??NCBI?EBI?CIB????,?????Ge
  nBank?EMBL?DDBJ???????????????????????????,???????
  ????????????,?????????????????

21
3???????

• ???,??????????????????????,???????????????????????
  ????,???????????????????
• ????????????,??????????????????????????(??????????
  ?),????????????????????,????,???????,?????????????

22
4??????????

• ??????????????????????????????????????????????????
  ??????????????????????????????????????????????????
  ????????????????????????????????????????(?????????
  ??????)???????????????????????????????????????????
  ?????????????????????????????????????????????????
  ???????????????????

23
4??????????

• ??????????????????????????????????????????????????
  ??????????????????????????????????????????????????
  ????????????????(???????????????)?????????????????
  ???????????????????????????EMBL???????,???????????
  ?,??????????http//www.Ipc.pku.edu.cn/mirror/mirro
  r.htmlhttp//www.Ebi.pku.edu.cn)????????????????
  ?????????????????????????????????????????????????
  ??????????

24
??????????DNA??????????,?????DNA????????,?????
?????????????????????????????????
25

• ?????????????????????????????????????????????
  ???????????????????????????????????????

26
????????

• ?????????1. ???? ??-gt??-gt??
• 2.??????????????????
• 3.?????
• ???????????????
• ???????????
• ???????????????????????????????????????

27
? ? ? ? ?

• ?????
• ????,Attwood T Kltlt???????gtgt??????????2002?4
• ??????????
• ??????????