An Overview

1
E. Coli Testing

• An Overview
• M. A. Peachie Cline Burlin, D.V.M.

2
RegulatoryRequirements

• Written plan
• Collect and analyze samples
• Records

3
Species Tested - Meat

• Cattle
• Swine
• Sheep
• Goats
• Horses
• Mules
• Other Equines

4
Species Tested - Poultry

• Chickens
• Turkeys
• Ducks
• Geese
• Guineas

5
Who Tests?

• PLANT PERSONNEL IN SLAUGHTER PLANTS

6
How?

• According to written plan
• FSIS guidelines
• FSIS video
• AOAC test

7
Record Keeping

• m/M values
• Statistical process control
• 13 test moving window
• Maintain 12 months

8
FSIS Responsibilities

• Basic Checklist
• Other Checklist
• Enforcement

9
Basic Checklist

• When?
• Special team 1/97
• Grant of inspection

Basic Checklist
1. Written plan 2. Sampling 3. Records
10
Other Checklist

• PBIS schedules

Other Checklist
1. Correct species 2. Location 3. Frequency 4.
Random selection 5. AOAC Method 6. Last 13
tests 7. CFU/ml or square cm 8. 12 months of
records 9. m/M value 10. SPC
11
Enforcement

• Withhold inspection
• Basic noncompliance
• Repetitive other noncompliance
• Shipping contaminated product
• Noncompliance Record
• Individual other noncompliance

12
The End