Introduction to Microscopy:

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Introduction to Microscopy

• The Light Microscope
• The TEM
• The SEM

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Introduction to Microscopy (Use of Microscopes to
study minute small objects)

• The image to the right is a light microscope
• Light microscopes bounce light waves off of an
  object. The light source may be from the sun or
  from a bulb from a lamp.
• The diaphragm of a light microscope controls the
  amount of light the enters the stage to
  illuminate the specimen being examined.

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Resolution The resolution of a microscope is a
measure of the clarity the image produced by the
scope the smallest distance between to points
that can be seen as being separate.

• http//www.olympusmicro.com/primer/java/microscopy
  /airydiscs/index.html
• The image below shows the difference between the
  resolution of a light microscope versus an
  electron microscope

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Electron Microscopes these microscopes bounce a
beam of electrons off of an object instead of
light. These microscopes have a high resolution
(smaller distance between two points)
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There are two types of electron microscopes

• SEM (scanning electron microscope)
• Produces a three-dimensional image)
• TEM (transmission electron microscope)
• Produces a two-dimensional image)

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SEM Images (Three-Dimensional)
http//micro.magnet.fsu.edu/primer/java/electronmi
croscopy/magnify1/index.html
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TEM images
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Which has better resolution of image?
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Controls amount of light entering the stage
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