MMP

1
MMPs and Their Role in the Wound

• Victor L. Sylvia, Ph.D.
• Associate Professor
• Department of Orthopaedics
• University of Texas Health Science Center
• 7703 Floyd Curl Drive
• San Antonio, TX 78220

2
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
3
(No Transcript)
4
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
5
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
6
MMPs and Growth Factors

• Pathological dysregulation of MMP activity
  results in excessive breakdown of the
  extracellular matrix (ECM) and loss of growth
  factors, involved in normal healing, that are
  stored in the ECM.
• Growth factors such as PDGF-BB, TGF-ß and VEGF
  are found in decreased amounts in chronic wounds,
  presumably due to the increase in MMPs (Loot et
  al., 2002 Lerman et al., 2003 Robson et al.,
  2004).

7
(No Transcript)
8
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
9
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
10
Repair
11
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
12
(No Transcript)
13
(No Transcript)
14
(No Transcript)
15
(No Transcript)
16
Matrix Metalloproteinases and their Substrates
MMP Synonyms EC Classification Latent M.W. (kDa) Active M.W. (kDa) Substrates
MMP-1 Interstitial Collagenase 3.4.24.7 55 45 Fibrillar collagens, casein, gelatin, proteoglycan
MMP-2 Gelatinase A, 72 kDa Type IV Collagenase 3.4.24.24 72 66 Denatured collagens, collagens IV, V, VII, X, gelatin
MMP-3 Stromelysin-1 3.4.24.17 57 45 Proteoglycan, collagens X, XI, procollagens, gelatin, laminin, collagenase, gelatinase B
MMP-7 Matrilysin PUMP 3.4.24.23 28 19 As stromelysin 1, elastin
MMP-8 Neutrophil collagenase 3.4.24.34 75 58 Fibrillar collagens, gelatin, proteoglycan
MMP-9 Gelatinase B, 92 kDa Type IV Collagenase 3.4.24.35 92 86 Denatured collagens, collagens IV, V, VII, X
MMP-10 Stromelysin 2 3.4.24.22 57 44 As Stromelysin 1
MMP-11 Stromelysin 3   62 47 Unknown
MMP-12 Macrophage metalloelastase 3.4.24.65 54 22 Elastin, gelatin, collagen IV, fibronectin, laminin, vitronectin, proteoglycan
MMP-13 Collagenase 3 -- 66 48 Fibrillar collagens, gelatin, aggrecan
17
(No Transcript)
18
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
19
Remodeling
20
(No Transcript)
21
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
22
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
23
(No Transcript)
24
http//www.med.ufl.edu/IDP/Core/Section205/IDP20
Grand20Rounds20Tissue20Engineering20200520ver
sion20320compressed.pdf
25
(No Transcript)
26
(No Transcript)
27
Effect of Nitric Oxide Donor SNAP on Human Skin
Fibroblasts
28
Effect of Nitric Oxide Donors on MMP-9 Expression
29
Effect of Nitric Oxide Donors on MMP-8 Expression
30
Effect of Nitric Oxide Donors on Wound Healing
31
Questions?
32
(No Transcript)