Chapter 6

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Chapter 6 Microbial Growth
Growth of Bacterial Cultures
Physical Requirements
Chemical Requirements
Culture Media
Measurement of Growth
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FINAL ROUND
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Topic 1 Physical Requirements 100 Question

• The optimum pH for most bacteria is
• a. 1
• b. 5
• c. 7
• d. 9

ANSWER
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3
Topic 1 Physical Requirements 100 Answer

• The optimum pH for most bacteria is
• a. 1
• b. 5
• c. 7 (correct answer)
• d. 9

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4
Topic 1 Physical Requirements 200 Question

• A psychrophile grows best at
• a. 37C
• b. 0C
• c. 25C
• d. 15C

ANSWER
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5
Topic 1 Physical Requirements200 Answer

• A psychrophile grows best at
• a. 37C
• b. 0C (correct answer)
• c. 25C
• d. 15C

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6
Topic 1 Physical Requirements 300 Question

• Organisms that require high salt concentrations
  for growth are called
• a. Halophiles
• b. Extreme halophiles
• c. Facultative halophiles
• d. Microhalophiles

ANSWER
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7
Topic 1 Physical Requirements 300 Answer

• Organisms that require high salt concentrations
  for growth are called
• a. Halophiles
• b. Extreme halophiles (correct answer)
• c. Facultative halophiles
• d. Microhalophiles

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8
Topic 1 Physical Requirements 400 Question

• The energy source utilized by producers in the
  hydrothermal vents on the ocean floor is
• a. Light
• b. Sulfur
• c. Organic molecules
• d. Carbon dioxide

ANSWER
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9
Topic 1 Physical Requirements 400 Answer

• The energy source utilized by producers in the
  hydrothermal vents on the ocean floor is
• a. Light
• b. Sulfur (correct answer)
• c. Organic molecules
• d. Carbon dioxide

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10
Topic 1 Physical Requirements 500 Question

• In food preservation, salts and sugars are used
  because they create a
• a. Lower pH
• b. Hypertonic environment
• c. Hypotonic environment
• d. Lower osmotic pressure

ANSWER
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11
Topic 1 Physical Requirements 500 Answer

• In food preservation, salts and sugars are used
  because they create a
• a. Lower pH
• b. Hypertonic environment (correct answer)
• c. Hypotonic environment
• d. Lower osmotic pressure

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12
Topic 2 Chemical Requirements100 Question

• Chemoautotrophs and photoautotrophs derive their
  carbon from
• a. Carbon dioxide
• b. Proteins
• c. Lipids
• d. Carbohydrates

ANSWER
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13
Topic 2 Chemical Requirements 100 Answer

• Chemoautotrophs and photoautotrophs derive their
  carbon from
• a. Carbon dioxide (correct answer)
• b. Proteins
• c. Lipids
• d. Carbohydrates

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14
Topic 2 Chemical Requirements 200 Question

• Some microorganisms use gaseous nitrogen directly
  from the atmosphere in a process called
• a. Chemosynthesis
• b. Photosynthesis
• c. Nitrogen fixation
• d. Nitrogen synthesis

ANSWER
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15
Topic 2 Chemical Requirements 200 Answer

• Some microorganisms use gaseous nitrogen directly
  from the atmosphere in a process called
• a. Chemosynthesis
• b. Photosynthesis
• c. Nitrogen fixation (correct answer)
• d. Nitrogen synthesis

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16
Topic 2 Chemical Requirements 300 Question

• An organism that uses oxygen but can
• grow without oxygen is called a(n)
• a. Obligate aerobe
• b. Facultative aerobe
• c. Aerobe
• d. Aerotolerant anaerobe

ANSWER
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17
Topic 2 Chemical Requirements 300 Answer

• An organism that uses oxygen but can
• grow without oxygen is called a(n)
• a. Obligate aerobe
• b. Facultative aerobe (correct answer)
• c. Aerobe
• d. Aerotolerant anaerobe

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18
Topic 2 Chemical Requirements 400 Question

• This organism is killed by atmospheric
• oxygen
• a. Aerobe
• b. Obligate aerobe
• c. Obligate anaerobe
• d. Aerotolerant anaerobe

ANSWER
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19
Topic 2 Chemical Requirements 400 Answer

• This organism is killed by atmospheric oxygen
• a. Aerobe
• b. Obligate aerobe
• c. Obligate anaerobe (correct answer)
• d. Aerotolerant anaerobe

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20
Topic 2 Chemical Requirements 500 Question

• Biofilms reside in a matrix made up primarily of
• a. Lipids
• b. Hydrogel
• c. Proteins
• d. Polysaccharides

ANSWER
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21
Topic 2 Chemical Requirements 500 Answer

• Biofilms reside in a matrix made up primarily of
• a. Lipids
• b. Hydrogel
• c. Proteins
• d. Polysaccharides (correct answer)

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22
Topic 3 Growth of Bacterial Cultures100
Question

• Generation time can best be defined as
• a. The length of time it takes for lag phase
• b. The duration of log phase
• c. The length of time it takes for a cell to
  divide
• d. The minimum rate of doubling

ANSWER
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23
Topic 3 Growth of Bacterial Cultures 100 Answer

• Generation time can best be defined as
• a. The length of time it takes for lag phase
• b. The duration of log phase
• c. The length of time it takes for a cell to
  divide (correct answer)
• d. The minimum rate of doubling

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24
Topic 3 Growth of Bacterial Cultures 200
Question

• To cultivate anaerobic bacteria, one must use
  special media called
• a. Reducing media
• b. Oxidizing media
• c. Differential media
• d. Selective media

ANSWER
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25
Topic 3 Growth of Bacterial Cultures 200 Answer

• To cultivate anaerobic bacteria, one must use
  special media called
• a. Reducing media (correct answer)
• b. Oxidizing media
• c. Differential media
• d. Selective media

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26
Topic 3 Growth of Bacterial Cultures 300
Question

• Capnophiles must be grown in high concentrations
  of
• a. Oxygen
• b. Salt
• c. Glucose
• d. Carbon dioxide

ANSWER
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27
Topic 3 Growth of Bacterial Cultures 300 Answer

• Capnophiles must be grown in high concentrations
  of
• a. Oxygen
• b. Salt
• c. Glucose
• d. Carbon dioxide (correct answer)

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28
Topic 3 Growth of Bacterial Cultures 400
Question

• The culture medium that is designed to increase
  numbers of desired microbes to detectable levels
  is called
• a. Complex
• b. Selective
• c. Enrichment
• d. Differential

ANSWER
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29
Topic 3 Growth of Bacterial Cultures 400 Answer

• The culture medium that is designed to increase
  numbers of desired microbes to detectable levels
  is called
• a. Complex
• b. Selective
• c. Enrichment (correct answer)
• d. Differential

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30
Topic 3 Growth of Bacterial Cultures 500
Question

• The culture medium that is designed to suppress
  unwanted microbes while encouraging desired
  microbes is called
• a. Complex
• b. Selective
• c. Reducing
• d. Differential

ANSWER
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31
Topic 3 Growth of Bacterial Cultures 500 Answer

• The culture medium that is designed to suppress
  unwanted microbes while encouraging desired
  microbes is called
• a. Complex
• b. Selective (correct answer)
• c. Reducing
• d. Differential

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32
Topic 4 Culture Media100 Question

• To neutralize acids as they are produced in a
  culture, what should be added to the culture
  medium?
• a. Buffers
• b. Salts
• c. Sugars
• d. PABA

ANSWER
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33
Topic 4 Culture Media100 Answer

• To neutralize acids as they are produced in a
  culture, what should be added to the culture
  medium?
• a. Buffers (correct answer)
• b. Salts
• c. Sugars
• d. PABA

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34
Topic 4 Culture Media 200 Question

• The microbes that grow and multiply in or on a
  culture medium are referred to as a(n)
• a. Culture
• b. Inoculum
• c. Specimen
• d. Medium

ANSWER
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35
Topic 4 Culture Media 200 Answer

• The microbes that grow and multiply in or on a
  culture medium are referred to as a(n)
• a. Culture (correct answer)
• b. Inoculum
• c. Specimen
• d. Medium

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36
Topic 4 Culture Media 300 Question

• What can be added to a medium when it is
  desirable to grow bacteria on a solid medium?
• a. Glucose
• b. Magnesium sulfate
• c. Potassium phosphate
• d. Agar

ANSWER
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37
Topic 4 Culture Media 300 Answer

• What can be added to a medium when it is
  desirable to grow bacteria on a solid medium?
• a. Glucose
• b. Magnesium sulfate
• c. Potassium phosphate
• d. Agar (correct answer)

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38
Topic 4 Culture Media 400 Question

• A culture medium consisting of agar, human blood,
  and beef heart is a
• a. Complex medium
• b. Selective medium
• c. Differential medium
• d. Chemically defined medium

ANSWER
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39
Topic 4 Culture Media 400 Answer

• A culture medium consisting of agar, human blood,
  and beef heart is a
• a. Complex medium (correct answer)
• b. Selective medium
• c. Differential medium
• d. Chemically defined medium

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40
Topic 4 Culture Media 500 Question

• The source of nutrients in nutrient agar is
• a. Agar
• b. Peptone and sodium chloride
• c. Peptone and beef extract
• d. Nutrient

ANSWER
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41
Topic 4 Culture Media 500 Answer

• The source of nutrients in nutrient agar is
• a. Agar
• b. Peptone and sodium chloride
• c. Peptone and beef extract (correct answer)
• d. Nutrient

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42
Topic 5 Measurement of Growth100 Question

• A spectrophotometer can be used to measure
• a. Metabolic activity
• b. Turbidity
• c. Dry weight
• d. Pathogenicity

ANSWER
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43
Topic 5 Measurement of Growth100 Answer

• A spectrophotometer can be used to measure
• a. Metabolic activity
• b. Turbidity (correct answer)
• c. Dry weight
• d. Pathogenicity

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44
Topic 5 Measurement of Growth 200 Question

• Because of time considerations, which method is
  usually used to count the number of bacteria in
  milk?
• a. The direct microscope count
• method
• b. The pour plate method
• c. The spread plate method
• d. The most probable number method

ANSWER
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45
Topic 5 Measurement of Growth 200 Answer

• Because of time considerations, which method is
  usually used to count the number of bacteria in
  Pasteurized milk?
• a. The direct microscope count
• method (correct answer)
• b. The pour plate method
• c. The spread plate method
• d. The most probable number method

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46
Topic 5 Measurement of Growth 300 Question

• Which method is utilized to ensure that plates
  containing 25 to 250 colonies are counted?
• a. The most probable number method
• b. Serial dilution
• c. Filtration
• d. Spread plate method

ANSWER
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47
Topic 5 Measurement of Growth 300 Answer

• Which method is utilized to ensure that plates
  containing 25 to 250 colonies are counted?
• a. The most probable number method
• b. Serial dilution (correct answer)
• c. Filtration
• d. Spread plate method

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48
Topic 5 Measurement of Growth 400 Question

• Microorganisms are particularly sensitive to
  adverse conditions during the
• a. Lag phase
• b. Death phase
• c. Stationary phase
• d. Log phase

ANSWER
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49
Topic 5 Measurement of Growth 400 Answer

• Microorganisms are particularly sensitive to
  adverse conditions when optimal growth occurs
  during the
• a. Lag phase
• b. Death phase
• c. Stationary phase
• d. Log phase (correct answer)

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50
Topic 5 Measurement of Growth 500 Question

• Which of the following metabolic activities can
  be used to measure microbial growth?
• a. Carbon dioxide consumption
• b. Agar consumption
• c. Glucose consumption
• d. Salt consumption

ANSWER
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51
Topic 5 Measurement of Growth 500 Answer

• Which of the following metabolic activities can
  be used to measure microbial growth?
• a. Carbon dioxide consumption
• b. Agar consumption
• c. Glucose consumption (correct answer)
• d. Salt consumption

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52
FINAL ROUND Question

• A serial dilution of 110,000 was made yielding
  a plate count of 26. What is the original
  bacteria count?
• a. 26
• b. 52
• c. 2,600
• d. 260,000

ANSWER
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53
FINAL ROUND Answer

• A serial dilution of 110,000 was made yielding a
  plate count of 26. What is the original bacteria
  count?
• a. 26
• b. 52
• c. 2,600
• d. 260,000 (correct answer)

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