Lactose Intolerance

1
Lactose Intolerance

• iGEM Meeting 4
• 07/16/08
• Presentation by Robert Ovadia

2
System Design
3
System Design
4
Last Weeks ResultsWhat we had
We have sequenced
We have transformed
LacY
LacZ
34
Into pSB1A2
LacY
Purified ready to cut
From PCR
Lysis Cassette
LacY
31
PCR Purified
31
33
LacY
33
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Last Weeks ResultsSequencing The Work Chart
Did it work?
?
LacY
31
?
LacY
33
?
LacZ
34
?
Lysis Cassette
6
Future GoalsWhats next?
LacZ
34
In J23100 Str. Po.
Lysis Cassette
In pSB1A2
In pSB2K3
LacY
31
In pSB1AK3
LacY
33
In pSB1AK3
7
Current Constructs
We have sequenced
Preparing to digest

• A whole lot of stuff
• Three promoters
• Lac. inducible promoters (A81, B4)
• RBS, GFP ,TT
• Our current constructs
• B0015 (1AK3 2K3)

LacY
LacY
31
LacY
33
LacZ
34
Lysis Cassette