Gut Microbes Lactose Intolerance

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Gut MicrobesLactose Intolerance
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Introduction
Our Goals

• From last week, we had three possible ways to
  tackle lactose intolerance
• Convert H2 or methane into less harmful gases
• Make a super sugar metabolizing machine
• Engineer a strain to NOT convert glucose
  galactose into H2 and methane.

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Goal 2
Super Sugar Metabolizing Machine

• In order for our strain of E. coli to consume
  lactose, we must force it.
• In one of our earlier meetings, Doug spoke about
  his Healthbots, which included the
  phosphoenolpyruvatesugar phosphotransferase
  system (PTS).

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Goal 2
Super Sugar Metabolizing Machine

• To review, some bacteria have a prevelance for
  certain sugars, mainly glucose, fructose and
  sucrose (PTS sugars).
• To save energy, they will not consume non PTS
  sugars (lactose, maltose, arabinose), if PTS
  sugars are present (known as Carbon Catabolite
  Repression).
• We must toggle the PTS by inhibiting the blocking
  of LacY.

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Goal 2
Super Sugar Metabolizing Machine
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Goal 2
Super Sugar Metabolizing Machine

• To have our strain uptake lactose, we could
  introduce specific mutations in LacY.
• This will hopefully prevent the binding of
  EIIA(GLUC), allowing our strain to uptake
  lactose.
• In addition, we can overexpress LacY to help the
  process.

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Goal 2
Super Sugar Metabolizing Machine
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Reference

1. Hoischen C, Levin J, Pitaknarongphorn S, Reizer
   J, and Saier MH Jr. Involvement of the central
   loop of the lactose permease of Escherichia coli
   in its allosteric regulation by the
   glucose-specific enzyme IIA of the
   phosphoenolpyruvate-dependent phosphotransferase
   system. J Bacteriol 1996 Oct 178(20) 6082-6.
   pmid8830713. PubMed HubMed Hoischen1996
2. Deutscher, J., Francke, C. and Postma, P.W. 2006.
   How Phosphotransferase System-Related Protein
   Phosphorylation Regulates Carbohydrate Metabolism
   in Bactertia. Microbial and Molecular Biology
   Reviews. 2006 939-1031