??????(Plant tissue culture)??????????,???????????????????????????????????????????

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• ??? ? ?
• ???????????
• 1. ? ?
• ??????(Plant tissue culture)??????????,????
  ???????????????????????????????????????

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2.???? (1)???????? (2)??????,?????????
??????????? (3)????????????????
????????????????????,?????????
(4)????????????????? (5)?????????????????,??
??????????????
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???????????????????????????? 1???????????
(1)??????(Plant Culture)?????????????
(2)????(Embryo Culture)???????????????????
(3)????(Organ Culture)???????????????????????
(4)????(Tissue Culture)??????????????????
(5)????(Cell Culture)?????????????????
(6)??????(Protoplast Culture)????????????????????
?
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???????????
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??????????????? Mature
embryo culture and rapid propagation of tree peony
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(No Transcript)
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2????????? (1)??????(Organogenesis)
??????????????????????????????
?????????????????(dedifferentiation)????(rediffer
entiation)??????????????????????
(2)???????(Somatic embryogenesis)
?????????????????????????.????????????????????????
???????????????????????????????(embryoid)?????(som
atic embryo).
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?????? ??????
???????
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??????????????
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?????????
????? ???
1??
5??
?BAP
BAP 0.1mg/l BAP
5.0mg/l ?NAA
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????? ???
1??
5??
?BAP
BAP 0.1mg/l BAP
5.0mg/l ?NAA
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????????
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??????(??????)
??????????????
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????????????
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???????????
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(No Transcript)
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????(Artificial seed,Synthetic Seed)???????????
???????????????????????????????,??????????????????
???
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3??????????? (1)????(Solid culture)
?????????? (2)???????(Semisolid
Culture)????? (3)????(Liquid
Culture)???????????
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??????? A ??????,?????? B ?????? C
???????
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????
????
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??????
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??????????
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??????
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??????????
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???????
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??????????????? ????????(Plant cellular
totipotency) ?????,????????????????????????
,???????????,???????????????
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(No Transcript)
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?????????????????????,????????????????????,?
????????? ????????,???????????????????????????
??????????,???????????????????????????????????????
??????????????? ???????????????????????,??????
?????????????????????
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• ???????????????
• 1? ?????? (1930???)
• 1893?,Schwann?Scheiden?????????????????
• 1902?,????????Haberlandt???????????????????
  ?????????????????????????,????????
• 1904?,Hanning????????????????????
• 1922?,Knudson????????????????????,Knotte?Ro
  bbins???????????Robbins??????????????????,????????
  ????
• 1925?,Laibach?????????????????????,??1929???
  ????????????????

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2??????????????(1930-1960?)
1933?,?????????????????,????????????????????
1937?,White??B??????????????????IAA???????????????
? 1937,1938?,Gantheret?????????????,???????????
??????????Nobecomt???????????????????????????
1948?,Skoog??????????????,??????????????/?????????
1950?,Ball?????????????????
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1952?,Morel?Martin??????????????????,??,??????????
? 1953?,Tuleche????????????????????? 1955?,Miller?
???????????????????,?????????????????????,???????
??3??? 1957?,Skoog?Miller?????????/?????,?????????
?? 1958?,Maheshwari?Rangaswamy????????????????????
???,Reinert ?Steward???????????????????????,?????
?????????????????????,?????????????????
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3????????????(1960???) 1960?,Morel????????????????
? 1962?,Murashige?Skoog???????Murashige?Skoog???
(MS?????),??????????????? 1964?,???Guha?Maheshwari
????????????????????????????????? 1971?,Takebe????
?????????????? 1972?,Carlson??????????????????????
? 1974?,Murashige?????????????????Zaenen?Larebeke?
????????(Agribacterium)????????????Ti??? 1978?,Mel
chers?????????????????
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1979?,Marton???????????????????????????? 1981?,Lar
kin?Scowcroft??????????(Somaclonal
variation)????? 1982?,Krens??????,???????????DNA,
???????DNA???????????? 1982?,Zimmermann???????????
??? 1985?,Horsch?????????????????,???????????
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?????????????

• 1????????????
• (1)??????????????????

???????????
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??????
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A
B
C
E
F
G
?????
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????
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????
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???????
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?????
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???????
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????????
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(No Transcript)
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(No Transcript)
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(2)????????????????????? ???????????
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virus-free tissue
??????
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????????????????
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2????????? ?????????????????????????????????
???????????????????????????????????????????????
??????
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3????????????????????????
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????????, ?????
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4?????????????,????????????????????????
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2005?9?6?