Chromatography I: Paper

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Chromatography I Paper
General Chemistry 101/102Laboratory
ManualUniversity of North Carolina at Wilmington
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Chromatography I Paper

• Purpose

• Use the technique of paper chromatography to
  separate a homogeneous mixture into its
  individual components.

• Safety Considerations

• Butanol and ammonia (both liquid and vapor) are
  toxic and are strong skin and eye irritants.
  Keep the ammonia in the hood and cover all
  butanol and ammonia solutions.
• Dispose of butanol in the waste container in the
  hood.

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Chromatography I Paper

• Procedure

• Place a small spot of each indicator on the line.

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Chromatography I Paper

• Procedure

• When the spots have dried, re-spot each one.

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Chromatography I Paper

• Procedure

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Chromatography I Paper

• Procedure

• Allow the chromatogram to dry before going to the
  next step.

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Chromatography I Paper

• Procedure

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Chromatography I Paper

• Procedure

• Use your computed RF values to identify the
  components of your unknown.