PowerPoint Wageningen UR

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NanoBioTecnologie alla Bicocca Stato dellarte
e future applicazioni
Giancarlo Baldini Maddalena Collini Laura
DAlfonso Giberto Chirico
Chiara Bosisio Michele Caccia Fabio
Cannone Valentina Quercioli
Uni-Parma A. Mozzarelli S. Bettati
B. Campanini
Uni-Viterbo S. Cannistraro A. Bizzarri
MDM G. Tallarida
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nanodevices
Molecular electronics
Artificial tissues Bio-sensors New probes New
drugs
Mosfet nanotubes transistors Nano-photo-voltaic
cells .
nanotechnologies
Nano-Manufacturing FIBS Focus Ion Beam
Spectroscopy AFM Dip Pen Nanolithography SEM
Scanning Electron Microscopy . CHEMISTRY!!!!
Biology new-tools Engineered proteins Diagnosis
Methods based on nanoparticles Therapeutic
effects of nanoparticles.
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Bottom-up technology self-assembled systems
Top-down technology
Layer by layer deposition up to 20000 processes
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? New Materials
Nanotubes fullerens
1985
1991-1992
Quantum dots
Synthetic materials
Conductors or semiconductors Very good heat
conductance High stiffness
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Why Nanobio?
Intrinsic Bottom-up technology
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Why NanoBio.?
Example from Nature
A mixed structure with disordered crystalline
domains ? unique elasticity and robustness
Like Steel with 1/50 density
Young Modulus 1.5 -1. 7 GPa !!
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Proteins ? 1-10 nm at most DNA
? 2 nm Quantum dots ? 1-10 nm Colloids ?
1-200 nm Nanotubes ? 1 2 nm
size
DNA is a self-replicating system DNA interactions
? complementary structures Proteins sensitive to
the nano-environment DNA and proteins charge
transfer Protein-protein or
protein-nanoparticles engineering
Self assembling
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Applications and pespectives
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Protein based devicesdye based devices
Fluorescence signal can be voltage-gated
in amplitude in dynamic response
(blinking) in color emission (switching)
GFP mutants
Fluorescein derivatives
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Protein based devices

• GFP mutants

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Switching between chemical states
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Unfolding-refolding cycles of the same GFP
molecules stability and memory of previous
unfolding cycles
GuHCl 5.3 M
Chirico et al. Biophys.J. 2003
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GFP oscillations near unfolding ? 440, 730
925 Hzemission collected in channels A and N
79 Single GFP
Anionic emission
Neutral emission
Green Anionic
Red Neutral
Baldini, Chirico, Cannone Science, 2005
Frequency Distributions
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GFP oscillations under a. c. electric or sound
fields R resonant field
Molecule 2
Molecule 64
R
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Protein emission modulation

• Frequencies in the kHz range
• Sensitivity to electric and acoustic fields
• Search for mutants..

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dye based sensors

• Fluorescein derivatives

Gold colloid
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Probing the Dye-gold plasmon interactions
Excited state lifetime non-radiative coupling
to the gold nanoparticle
Fluorescence Blinking local field effect
Free Samsa
IRF
Excited state lifetime 4 ?0.4 ns
Blinking frequency 0.1 ?1 Hz
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Vacuum Level
S2
S1
LUMO
2.6 eV
S0
HOMO
dye
Gold NP
Cannone et al. JPC-B, 2006
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Voltage regulation of blinking
SAMSA on 5 nm gold colloids
E 0V/cm
E 10V/cm
E 15V/cm
E 20V/cm
2 mm
n 1 Hz
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Nano-bio-technologies?

• Manipulation of objects at the atomic scale
• - Dimensions not exceeding 100 nanometers
• Bottom up assembly DNA-proteins
• Billion research funding ??
• Estimated multi billio market for products ??
• Estimated ?? researcher in the field??