Aucun titre de diapositive

1
ANALYSIS OF GELS AND BLOTS
Bioprint 1D Main Photoshop Powerpoint
For more information Dr. Beat RIEDERER
Tel. [phone]
Where is it 2e Sous-sol 031
"Always" accessible
BeatMichel.Riederer_at_ibcm.unil.ch
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ANALYSIS OF GELS AND BLOTS
Set up
Light and UV Table
Lamps
Camera CCD, monochrome 434'000 pixels
Video Copy Processor (Mitsubishi, Mod.P68E)
Pentium Computer
Programs
Bioprint 1D Main
Some Applications
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PLASTINATED TISSUE
Transverse sections through the right thigh.
Transverse sections through the right leg.
Frontal section through the right foot
For more inforation on plastination
http//www-ibcm.unil.ch/teaching/plast/index.htm
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This is an agarose gel with samples of a mRNA
preparation
Miniprep
Separation of RNA and DNA. Detection by UV light.
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IEF
GFAP
SDS-PAGE
Identification of proteins and their modifications
Two Dimensional Gels
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WESTERN BLOTS
Observe the difference in GFAP isoforms in
Alzheimer  disease
young
old
GFAP in Human Brain (entorhinal cortex)
Alzheimer
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1D Main
Image Analysis
Scan import, TIFF
Quantification
Identification
Comparison
Molecular weights
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Example Quantification of spots
1D Main
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This program is used in Geneva, and serves to
identify differences in the protein composition
of 2D gels
You can learn it in 10 minutes! Do you believe it?