Biological Nanobots

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Biological Nanobots
Wayne Materi, PhD. National Institute for
Nanotechnology
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Mature nanotechnology will have many of the
characteristics, challenges and problems of
living organisms.
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Passive materials constant physico-chemical
properties
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Active materials Variable physico-chemical
properties
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• nanobots are introduced into patients
  bloodstream
• move towards atherosclerotic plaque
• bind in the region
• make and secrete a plaque-dissolving protein (
  ApoA-I(M) )
• exhibit behavioural dependency

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Falke and Hazelbauer, 2001
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Trivedi and Spudich, 2003
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Looger et al., 2003
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• tactic response cant interact with external
  protein
• tactic response does not activate transcription
• connecting regulation

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• demonstrate complex programmable behaviour in E.
  coli
• develop some novel sensory systems
• develop some autonomous regulation mechanisms
• explore novel exogenous regulation mechanisms
• develop techniques for routine construction of
  genetic networks

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• Protein design
• Identify candidate mechanisms
• Rationale design Directed evolution
• External control (Left-handed sugars /
  Right-handed amino acids)
• Status communication (Secreted peptides/small
  molecules / GFP)
• Internal logic
• Integrate engineered genes into chromosome
• Limit growth
• Limit immune response

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• Chromosomal Integration
• Directed evolution with feedback

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David Wishart Shannon Lubitz Valentyna
Semenchenko Members of E. coli community