Leica SP2

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Leica SP2
Introduction to the Microscopy Suites new multi
photon confocal microscope Glenn Fried
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Leica SP2
Outline

• Instrumentation
• Optical sectioning
• Spectral scans
• Multi photon microscopy

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Leica SP2
UV
Vis
NIR
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Leica SP2
UV
Vis
NIR
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Leica SP2
UV
Vis
NIR
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Leica SP2
Optical sectioning
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Leica SP2
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Leica SP2
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Leica SP2
Wavelength scan
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Leica SP2
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Leica SP2
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Leica SP2
Multi photon imaging
energy
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Leica SP2
Single photon
Two photon
Nsp
Nsp2
s1x10-16
s1x10-50
Use

• Short high intensity pulses
• focused

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Leica SP2
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Leica SP2
Funding provided by
The Beckman Institute for Advanced Science and
Technology Paul Braun Jeff Moore
Jennifer Lewis Steve Sligar Gerard
Wong Stephen Boppart Bill Greenough Jonathan
Sweedler Materials Science and
Engineering School of Chemical Science Chemical
Engineering Chemistry
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Leica SP2