History of Leica Confocal Microscope Systems

1
History of Leica Confocal Microscope Systems
2
1988 Wild Leitz CPM
Confocal Profile Measurement System for
Semiconductor industry

• Reflection mode
• Fully automated
• Critical Dimension
• measurements
• Profile measurements
• VME workstation/OS-9

3
1989 Wild Leitz CLSM
Confocal Fluorescence/Reflection System for
Biomedical and Industrial Applications

• High resolution
• focusing stage
• Control panel
• Single scan mirror
• Computer- controlled
• confocal pinhole
• VME workstation/
• OS-9
• 3D reconstruction

4
1989 Wild Leitz CLSM
High resolution focusing stage

• live vertical sectioning
• reproduceability 40 nm

5
1990 Wild Leitz CLSM Fluovert
Biomedical Confocal System with Research Inverted
Microscope Wild Leitz Fluovert

• Compact scanner module
• integrated in anti- vibration table

6
1991 Wild Leitz CLSM Fluovert
First commercial Confocal System with fiber
delivery of visible range lasers and UV Laser

• alignment-free
• more compact
• UV Version

7
1992 Leica CLSM Aristoplan
Compact scanner module on upright research
microscope Leica Aristoplan

• 1992 Windows-
• based user interface
• 1994 Physiology SW
• 1995 Multi color SW

8
1992 Leica TCS 4D
Redesigned compact optics for Leica DM R
microscopes

• High efficiency
• ultra-stable
• 4 fluorescence channels
• new transmitted light
• detector

9
1993 AOTF
Acousto-optical tunable filter for confocal
microscopy

• Free selection of excitation lines
• Individual line attenuation
• Less crosstalk
• Less fading

10
1993 Leica TCS 4D
System interchangeable between upright and
inverted Leica DM R series microscopes

• Flexibility for multi-disciplinary
• use

11
1993 UV fiber coupling

• vibration isolation
• heat isolation
• alignment-free

12
1995 PMT cooling
Active photomultiplier cooling

• increased sensitivity
• reduced dark noise

13
1996 Leica TCS NT
New Electronics and Software

• Entirely PC-based
• Windows NT
• Guiding user interface
• Online tutorial
• Time Lapse SW
• 2.5 D reconstruction
• 3D reconstruction
• Physiology SW

14
1998 Leica TCS MP
Multi Photon Microscope

• Single and multi-
• photon modes
• in one system
• Non-descanned
• detectors
• Fiber-coupled
• lasers,
• alignment-free
• Easy
• wavelength
• tuning

15

1999 LCS
Leica Confocal Software

• Optimised for low light laboratory
  conditions
• Easy to use user interface minimizes learning
  time
• Built-in multidimensional imaging core
  optimised for 3D, 4D, 5D, 6D, 7D
  applications.
• Burst mode for real-time response of
  time-critical functions
• Digital potentiometer support for quick
  instrument set-up
• Modular and configurable System Control Layer
• Open system based on MS-DCOM technology
• Supports complex scan processes
• Macro recording and playback
•  

16
2000 Leica TCS SP2
Spectral Confocal and Multiphoton Microscope

• Superior Image performance
• MP, VIS, UV in one system
• K-Scanner for high resolution and
  fast scanning
• Adjustable pupil illumination for best
  image quality
• Up to 4096x4096 pixel resolution
• Advanced scan modes