Identifying Bacteria In Zebrafish

1
Identifying Bacteria In Zebrafish

• Caitlin Dickens

2
Purpose

• To identify the bacteria which is infecting a
  particular zebrafish

3
Hypothesis

• If bacteria are able to grow on plates selecting
  for mycobacteria, then the bacteria are likely to
  be mycobacteria because the antibiotics should
  inhibit the growth of all other bacteria.

4
Procedure
5
Results

• Aeromonas sp. m22 16S ribosomal RNA gene, partial
  sequenceLength1509 Score 693 bits (768),
  Expect 0.0Identities 388/399 (97), Gaps
  1/399 (0)StrandPlus/Plus 
• Query 10 TTAGAGTTTGATC-TGGCTCAGATTGAACGCTGGCGGCAG
  GCCTAACACATGCAAGTCGA 68
• 
  
• Sbjct 2TTAGAGTTTGATCCTGGCTCAGATTGAACGCTGGCGGCAGGC
  CTAACACATGCAAGTCGA 61 
• Query 69 GCGGCAGCGGGAAAGTAGCTTGCTACTTTTGCCGGCGA
  GCGGCGGACGGGTGAGTAATGC 128
  
• Sbjct 62 GCGGCAGCGGGAAAGTAGCTTGCTACTTTTGCCGGCGA
  GCGGCGGACGGGTGAGTAATGC 121 
• Query 129 CTGGGAAATTGCCCAGTCGAGGGGGATAACAGTTGGAA
  ACGACTGCTAATACCGCATACG 188
  
• Sbjct 122 CTGGGAAATTGCCCAGTCGAGGGGGATAACAGTTGGAA
  ACGACTGCTAATACCGCATACG 181 
• Query 189 CCCTACGGGGGAAAGCAGGGGACCTTCGGGCCTTGCGC
  GATTGGATATGCCCAGGTGGGA 248
• 
  
• Sbjct 182 CCCTACGGGGGAAAGCAGGGGACCTTCGGGCCTTGCGC
  GATTGGATATGCCCAGGTGGGA 241 
• Query 249 TTAGCTAGTTGGTGAGGTAATGGCTCMCCAAGGCGACG
  ATCCCTAGCTGGTCTGAGAGGA 308
• 
  
• Sbjct 242 TTAGCTAGTTGGTGAGGTAATGGCTCACCAAGGCGACG
  ATCCCTAGCTGGTCTGAGAGGA 301 
• Query 309 TGATCAGCCMCACTGGAACTGAGMCACGGYCCAGACTC
  YTACGGGAGGCAGCAGKGGGRA 368
• 
  
• Sbjct 302 TGATCAGCCACACTGGAACTGAGACACGGTCCAGACTC
  CTACGGGAGGCAGCAGTGGGGA 361 
• Query 369 WTATTGCACARKGGGGGAAACCCTGATGCAGCCATGCC
  G 407

6
Results

• Zebrafish used for the specimin was infected eith
  Aeromonas.
• Part of the normal intestinal flora in healthy
  fish.
• Causes septicemia in infected fish.

7
Results

• The results of this experiment are inconclusive.