Practical Review Practice( SemI)

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Practical Review Practice( SemI)

• Advanced Placement Biology
• Laboratory Review Sheet

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Dissolved oxygen simulation

• http//www.phschool.com/science/biology_place/labb
  ench/lab12/varlight.html
• Play the simulation.
• Relate to an aquatic ecosystem
• How does dissolved oxygen relate to primary
  productivity
• How do the following variables affect dissolved
  oxygen
• Temperature
• Salt water and fresh water?
• Running and still

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Question and answers

• If the water temperature increases, the dissolved
  oxygen________
• Decreases
• In general running water has ________
• ( more or less) oxygen than still water
• More

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Nomogram
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Read the saturation

• For a temperature of 25oC, what is the
  saturation ?
• For a temperature of 25oC, what is the dissolved
  oxygen?

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Class Microscope. http//www.microscope-microscop
e.org/basic/microscope-parts.htm

• Identify the following
• Objective lenses
• Condenser
• Mechanical stage contraol
• Fine Adjustment
• Coarse Adjustment
• Rotating noewpiece
• Be able to identifythe following
• Oil
• High
• Low
• Scanning

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How to prepare a wet mount. What are these steps?
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What is this cell?
Starfish egg cell
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Is this cell a eukaryote or a prokaryote?
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What s the difference between these two pictures
of Spirogyra? How would Spirogyra be classified?
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What is Volvox?
Why does this show evolution?


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Spectrophotometer

• http//www.umd.umich.edu/casl/natsci/slc/spec21.sw
  f - simulation

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Spectrophotometer

• What is absorbance?
• What is percent transmittance?

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What colors are associated with the wavelengths
of visible light
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Chromatography with a Flair
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Chromatography

• What is the matrix?
• What is the solute?
• What is the solvent?
• What is the basis of separation?
• What is the Rf value?

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Gel electropohoresis
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What is an electrophoresis chamber ?
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Describe how to put the sample in the wells?
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The sample is loaded at this end of the gel box
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This shows the molecules moving toward the
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DNA gel
What is the stain in the picture? What is the
marker used with this experiment?
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DNA Electrophoresis
Which is the marker?
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Micromeasurement
What is the reading on this micropipet?
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Micropipet Use( review)
Review how to use the micropipet
http//www.fhcrc.org/education/hutchlab/lessons/us
e.html
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What is an isolation streak?
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What is flaming?
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What is the shape of these bacteria? Which one
is gram positive?
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What type of bacteria are these?
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What type of bacteria are these?
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What type of bacteria are these?
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Kirby Bauer
What is resistance , what is sensitivity?