Cell Fate Determination in the Nervous System

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Cell Fate Determinationin the Nervous System
Jeh-Ping (Serena) Liu jl7nf_at_virginia.edu
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Development of the Vertebrate Nervous System
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Location! Location! Location
Timing is Everything!
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Spinal Motor Neurons Located in the Ventral
Spinal Cord
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Functions of the Spinal Motor Neurons
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Sensorimotor circuits in the spinal cord
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Dorsoventral Patterning
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TGF-b/BMP Signaling Pathway
KOHEI MIYAZONO et. al., JOURNAL OF CELLULAR
PHYSIOLOGY 187265276 (2001)
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Shh Signaling Pathway
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Homeodomain Structure
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Grafted Notochord Induces Ectopic Floor Plate
and Motor Neurons
Yamada et. al., 1992
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Using Neural Plate Explants to Study Neural
Patterning in vitro
Yamada et. al., 1993
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Co-expression of SC1 and Isl-1 in Motor Neurons
SC1 Isl-1
Yamada et. al., 1993
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Notochord Induces Motor Neurons in vitro
Isl1
Not1
Yamada et. al., 1993
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Shh Expression in Notochord and Floor Plate
Riddle et. al., 1993
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Concentration-dependent Induction of Floor Plate
and Motor Neurons by Shh
Roelink et. al., 1995
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Concentration-dependent Induction of Different
Neuronal Fate by Shh
Briscoe and Ericson 2001
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Class I and Class II Transcription Factors
Defines Progenitor Domains in the Ventral Spinal
Cord
Shh
Kessaris et. al., 2001
Novitch et. al. 2001
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Targeted Mutagenesis Using ES Cell Technology
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Cross-repression between Class I and Class II
Genes Generate Different Progenitor Domains
Briscoe and Ericson 2001
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Transcription Control of Motor Neuron Generation
Jessell, T. Nature Reviews of Genetics 2000
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Rostrocaudal Patterning
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Rostrocaudal Organization of the Spinal Cord
dorsal
ventral
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Expression of LIM Homeodomain Proteins in Motor
Neurons
Tsuchida et. al., 1994
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Drosophila Homeotic Gene Complex
from Developmental Biology 6th Edition by S.F.
Gilbert
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Hox-c Protein Expression in the Spinal Cord of
HH Stage 24 Chick Embryos
Liu, J-P. et. al., Neuron 2001
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Expression Pattern of Signaling Factors
Liu et. al., 2001
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Expression Pattern of Signaling Factors
Liu et. al., 2001
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Combinatorial Effects of Signaling Factors
Determine Spinal Cord R-C Identity
Liu et. al., 2001
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Expression of Hoxc6 in Thoracic Levels Induces
Brachial and Represses Thoracic Motor Neuron
Identity
Dasen, J. et. al., Nature 2003
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Express Hoxc9 in Brachial Levels Induces
Thoracic and Represses Brachial Motor Neuron
Identity
Dasen, J. et. al., Nature 2003
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Local signaling centers pattern neural tube
Rubenstein, 1997
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Transcription Factor Expression in the
Embryonic Mouse Brain
Rubenstein, 1997
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Integration of D-V and R-C signals results in
different progenitor domains
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Timing is Everything!
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Temporal Component in Cell Fate Determination
Kessaris et. al., 2001
Zhou et al., 2001
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Transcription Factor Expression and Neuronal
Types in Dorsal Spinal Cord
Helms and Johnson, 2003
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Time of Birth Determines Cortical Neuron Identity
Bielas S. et. al. 2004
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Drosophila Neuroblast Formation
James B. Skeath BioEssays199921922931.
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Location of Neuroblasts in a Hemisegment
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Drosophila Neuroblasts Sequentially Express
Transcription Factors which Specify the Temporal
Identity of Their Neuronal Progeny
Isshiki et al., Cell, 2001
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RA induces LMCL Identity
Sockanathan and Jessell, 1998
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Neural Crest Cells and Peripheral Nervous
System
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Trainor, P., and Krumlauf, R. Science
2002 Garcia-Castro, MI. et. al., Science 2002
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Liu and Jessell, Development 1998
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Wg/Wnt Signaling Pathway
Joerg Huelsken and Walter Birchmeier Current
Opinion in Genetics Development 2001,
11547553
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Derivitives of Neural Crest at Different R-C
Levels
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Transplantation of Premigratory Neural Crest
from Developmental Biology 6th Edition by S.F.
Gilbert
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Local Environment Determines the Fate of Neural
Crest Progeny
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Is this the whole story?
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Requirement of Target-derived Signals in Defining
Transmitter Type
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