Seminars and posters

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Seminars and posters

• seminar organization and general guidelines
• audiovisual aids
• preparing talks
• a few slides
• curriculum vitae for presenters
• preparing posters
• (poster viewing)

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• Giving a seminar....

• www.marine.unc.edu/seminars/ howto.html\\\

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Organization of a Typical Seminar

• start with broad introduction, then narrow focus
• discuss topic in detail
• summarize and explain general implications

www.bellboycorp.com/supplies/ images/GLASS-MA0.JP
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Organization of a Typical Seminar

• start with broad introduction, then narrow focus
• discuss topic in detail
• summarize and explain general implications

www.bellboycorp.com/supplies/ images/GLASS-MA0.JP
G
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Organization of a Typical Seminar

• start with broad introduction, then narrow focus
• discuss topic in detail
• summarize and explain general implications

www.bellboycorp.com/supplies/ images/GLASS-MA0.JP
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Guidelines for oral presentations

• know audience, context
• develop story in logical fashion link sections
• concentrate concepts, avoid details
• (usually) deal with each theme as a unit
• present key ideas several ways
• kinna avoid, well sort of dont talk, I mean
  discuss things.

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Guidelines cont.d

• avoid cute/rude humour
• use simple A/V aids some variety is good
• practice (with people)
• attend seminars
• be yourself
• dont apologize

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Audiovisual Aids

• Blackboards
• good for sequential explanations, small rooms
• Overheads
• often adequate (use correct font, orientation)
• use correct type for printer/copier
• 35 mm Slides
• not common now (arguably best quality)
• Video projectors (PowerPoint)
• very common watch compatibility
• resist complexity!

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General Considerations

• choose simple, consistent style
• thumb rules for slides
• no more than 8-10 lines/slide
• use short phrases, lists
• simplify published tables, figures, label clearly
• animation may be appropriate for more complex
  figures/tables
• acknowledge the work of others

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Preparing Your Talk

• decide on topic (audience, time, context)
• write preliminary outline
• prepare figs., tables etc.
• write more detailed outline (1 slide/min.)
• prepare paper/computer copy of talk before
  committing to CD /web (Use spell check!!)
• prepare slides, disk etc. and backup (web)
• practice, practice, practice (people)

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The good, the bad, and the ugly
www.westernposterpage.com/ ugly3.htm
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Actinobacillus suis Pathogen or Opportunist?
Janet MacInnes, University of Guelph
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Outline

• Background
• Population structure in swine, other species
• Cell surface esp. LPS
• Challenge studies, serum resistance
• Conclusions
• Acknowledgements

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Actinobacillus suis

• Typical gram negative bacterium, member of the
  family Pasteurellaceae
• requires complex medium
• haemolytic
• oxidase, catalaze, urease positive
• grows on MacConkey agar
• Isolated from
• pigs of various ages
• variety of birds, mammals

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Serum resistance

• Question Can the differences in virulence of O1
  and O2 strains be explained by differences in
  serum resistance?
• Hypothesis A. suis O2 strains are more serum
  resistant.

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Virulence studies

• Animals
• three test groups (n7)
• one PBS control group (n3)
• challenge by IP route (107 CFU/ml)
• Strains used
• O1/K1 (SO4)
• O1/K2 (C84)
• O2/K2 (H91-0380)

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Prevalence of A. suis O types
2 equine weak O1 cat, dog O2
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Mean total histopathologic score
01/K1ltO1/K2ltO2/K2
Mean Total Score (?SD)
omentum gastric serosa
splenic mesentery
mesen- tery
bact. clumps
colonic serosa
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Serum Resistance Profiles
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Serum Resistance Profiles
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A. suis LPSs probed with O2 antisera

• 1. SO4
• 2 H93-0055
• 3. B49
• 4. C84
• 5. H91-0380
• 6. H89-1173
• 7. H92-2146
• 8. H90-2521

1 2 3 4 5 6 7
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(No Transcript)
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Genetic organization of 14.4. kbp A. suis K1
cluster
Region 2
Region 1
pDSG1
XbaI
XbaI
pDSG2
PstI
PstI
XhoI
XhoI
pDSG3
PstI
PstI
pDSG4
1 kb
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(C)PS genes of A. suis SO4 (O1/K1)
Export
intragenic region
235 bp
wzt
wzm
wzf
wza
modF
.
orf1
orf2
orf3
orf5
orf6
orf 7
orf4
lysA
Synthesis?
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Functional role of the left-end early genes of
fowl adenovirus 9
Bryan Griffin MSc student September 24, 2008
PABI6440 - examples of what not to do on your
slides (and how they were improved)
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Adenovirus
Before 1

• Nucleic acid
• dsDNA, 24-45 kb, linear
• Structure
• 70-90 nm
• Icosahedral symmetrey
• Non-enveloped
• Taxonomy
• Family
• Adenoviridae
• Genera
• Mastadenovirus
• Human adenovirus (HAdV)
• Aviadenovirus
• Fowl adenovirus (FAdV)
• Atadenovirus
• Siadenovirus

http//nobelprize.org/nobel_prizes/medicine/laurea
tes/1993/illpres/genes-in-pieces.html
Fig. Summary of FAdV sequences currently
deposited in Genbank.
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Adenovirus (background)

• Taxonomy
• Family
• Adenoviridae
• Genera
• Mastadenovirus
• Human adenovirus (HAdV)
• Aviadenovirus
• Fowl adenovirus (FAdV)
• Atadenovirus
• Siadenovirus
• Nucleic acid
• dsDNA, 24-45 kb, linear
• Structure
• 70-90 nm
• Icosahedral symmetrey
• Non-enveloped
• Major strucural proteins
• Fiber

http//nobelprize.org/nobel_prizes/medicine/laurea
tes/1993/illpres/genes-in-pieces.html
After 1a
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Adenovirus (background)

• Many human adenoviral genomes have been
  sequenced
• A (HAdV-12)
• B (HAdV-3, -7,-11,-35)
• C (HAdV-1, -2, -5)
• D (HAdV-9, -17)
• E (HAdV-4)
• F (HAdV-40)
• Some animal adenoviral genomes have been
  sequenced
• simian, canine, murine, bovine, ovine, porcine,
  snake, tree shrew, duck, turkey, fowl

After 1b
Fig. Summary of FAdV sequences currently
deposited in Genbank.
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Objectives
Before 3
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Objectives
After 3

• Long term objectives
• Determine the cellular functions of ORF1C and
  ORF1
• Do ORF1C- or ORF1- FAdV-9 correlate to the
  FAdV-9?4 in vivo phenotype?
• Short term objectives
• Demonstrate that ORF1C protein is expressed in
  cell culture
• Determine ORF1C-host protein interactions
• Screen for BPV E5-like activities
• Screen for novel interactions
• Determine the subcellular location of ORF1C
  protein in cell culture
• ER, Golgi, plasma membrane
• Characterize ORF1C structurally and determine the
  critical residues
• Dimerization
• Mutational analysis
• Demonstrate that ORF1 (dUTPase) protein is
  expressed in cell culture
• Determine the importance of ORF1 (dUTPase) in
  vivo.

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Curricula vitae

• styles vary greatly good books, web sites,
  university resources
• styles may be prescribed (e.g., CIHR)
• start a detailed academic curriculum vitae and
  keep it up to date (e.g. put in pages for meeting
  abstracts etc.)
• spelling (and other niceties) count!!!
• should be tailored to situation

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Typical CV sections (A reminder)

• contact information
• education (place, dates, advisor/topic)
• relevant job (and other experience)
• professional certification
• (academic) awards
• publications (refereed, non-refereed, abstracts)
• names of referees (ask first)
• memberships in academic or professional societies
  
• (hobbies, other)

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CVs for speakers

• sections most often used are
• education (place, dates, advisor/topic)
• professional certification
• (big academic awards)
• publications (refereed, non-refereed, abstracts)
• relevant job (and other experience)
• names of referees
• memberships in academic or professional societies
  
• (hobbies, other)

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Posters

• increasingly common at meetings
• format requirements vary widely
• usually printed on a single page
• size/orientation counts!
• title and other requirements e.g. poster number
  headings may be dictated
• prepared using PowerPoint, CoralDRAW
• do rough sketch, prepare elements and import
• laminated posters are more durable
• remember your tacks or Velcro tape....

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General guidelines

• do not be afraid of using large text
• titles 90 to 150 point in bold
• text 30 to 32 point, sans serif
• upper and lower case vs. all upper case best
• dark text on light easiest to read contrast
• point form often appropriate esp. for conclusions
• usually 2 to 4 columns (use grids/margins)
• aim for 40 graphic content
• figs/tables minimum 5 X 7 inches, 300 dpi
• consider colour of graphics/using contrasting
  blocks to set graphics off when choosing
  background and title colours
• graphic content can be 3D

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A couple of useful websites

• http//www.soe.uoguelph.ca/webfiles/agalvez/poster
  /
• http//www.tss.uoguelph.ca/ltci/TGuides/epdfinal.p
  df
• http//www.ovc.uoguelph.ca/its/printing.cfm