Buck Scientific, Inc.

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Buck Scientific, Inc.

• Seminar in ANALYTICAL INSTRUMENTATION for
  Spectroscopy Chromatography
• UV-Vis Spectrophotometry

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Ultra-Violet/Visible SPECTROSCOPY (UV-Vis)
Theory Principle of OPERATION

• A TYPE of VIBRATIONAL Spectroscopy, similar to
  Infra-Red. A Sample in a Cuvette is exposed to
  Light Energy between 190nm. (in the UV) and
  1000nm. (in the VIS). Certain parts of an
  Organic Molecule will ABSORB some of this energy
  to create PEAKS on a SPECTRUM for Quantitative
  (primarily) and Qualitative Analysis

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BASIC Spectrophotometer Design
Like AAS instrumentation, the ORIGINAL UV-Vis
Specs were made as DOUBLE-BEAM units to correct
for Noise, Drift and other Instabilities. Over
20 years ago, the well-known leaders in the
Analytical Instrument markets Beckman
Perkin-Elmer began to focus on a line of
Stable-Beam SINGLE-BEAM Instruments. The
BUCK/Cecil series employs this optimum design in
our instruments with unique Internal software
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Basic SPECTROMETER Design

• ALL Analytical Spectrometers have several BASIC
  design components in common
• Light Source
• Sample Chamber
• Optical System
• Detector
• Output

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Basic SPECTROMETER Design

• ALL Analytical Spectrometers have several BASIC
  design components in common
• LIGHT SOURCE External D2 W Lamps to prevent
• thermal instability within instrument (no fan)
• SAMPLE Chamber Standard Specialty Holders
• OPTICS High-energy Ebert Monochromator
• DETECTOR Choice of low-Noise Photo-Diode
  or high-Sensitivity Photo-Multiplier Tube
• OUTPUT Graphics On-Screen Display, Analog,
  Printer
• and Serial RS-232 P-C capabilities

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UV/Vis Important Features

• Scan Speed
• Resolution
• Software Features
• Ease of Operation
• Data Storage
• Customized Calculations
• Support Service

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BUCK/Cecil Instruments

• The COMPACT, Stable-Beam construction of the
  Single-Beam series uses the high-quality Cecil
  base instrument and high-performance components
  from BUCK Scientific

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BUCK/Cecil Instruments

• Best optical design and highest quality
  components resulting in superior specifications
• Stray Light
• Noise
• Stability
• Baseline Flatness
• Accuracy (wavelength photometric / ABS)
• Reproducibility (wavelength photometric / ABS)

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BUCK/Cecil Series

• Series 2000
• Single Beam, Scanning, 4nm bandpass
• Optional built-in printer P-C Interface
• Many software options
• Most POPULAR Industrial system
• Applications Bio-Quest, Aqua-Quest, Food-Quest
  series with Prep-Programmed Operations

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BUCK/Cecil Series

• Series 3000
• Single Beam, Scanning, 1.8nm bandpass
• Optional built-in printer P-C interface
• Many software features (Kinetics, Bio-Assay)
• Reflectance option 3055 w/ Integrating Sphere

Specifically designed to meet ALL of the required
Specifications for USP / BP Pharmaceutical Assays
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BUCK/Cecil Series

• Series 7000/7000/9000
• Double-Beam, Scanning, some Models with
• variable 0.5 / 1.0 / 1.8-2.0 / 4.0nm bandpass
• Standard Printer P-C interface, Data-Stream
• Many software features (Kinetics, Bio-Assay)
• Reflectance / Fluorescence / Turbidity options

Ideal for the stringent requirements of Academic
and Pharmaceutical Research
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Sample Handling Options

• Cuvette - standard cell holder
• Quartz for UV range
• Glass Plastic for Vis range
• 1-100mm Pathlengths
• Flow Cell - standard or micro flow (50?L)
• Uses Sipette Pump
• Optional Automatic Cell Changer

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Sample Handling Options

• Multi-Cell Changer
• 4, 6, or 8 Positions
• Manual, Automatic, Programmable
• Thermostatted Cell Holder
• Water-jacketted or Electronic Peltier system
• Batch Sampler - 40 Positions Carousel
• Specular Integrating Sphere / Diffuse
  Reflectance

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Sample Handling Options
Multiple position CELL CHANGERS, MICRO-SIPPERS
and Sequential Access BATCH SAMPLERS
Allow the Chemist complete FLEXIBILITY for
MAXIMUM Sample Throughput Volume on ANY of the
BUCK/Cecil UV-Vis Spectrophotometers
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Software Features
A convenient INTERNAL Ink-Printer, and several
P-C Software Programs provide many Data
Processing and Manipulation options for
Qualitative Quantitative Work
Economical UV-Scan and sophisticated GRAMS/UV
Data-Stream are available
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Software Features
The INTERNAL Software built-in to the BUCK/Cecil
UV-Vis Spectrophotometers is capable of
performing General Analytical, Environmental,
Biotechnology and Clinical Assay WITHOUT the need
for a Computer

• Christian-Warburg Protein / DNA Measurements
• Colorimetric Anion Calibrations stored in the
  instrument
• Wavelength Subtraction, Spectral Overlay
  Smoothing,
• Derivatization and auto Peak-Pick on all systems
• Multi-Component Analysis, with 1o / 2o and 3o
  Polynomial
• Calibrations with up to 10 Standards with
  Statistics

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APPLICATIONS

• Primarily used for QUANTITATIVE Analysis of Known
  Compounds, UV-Vis is one of the most popular
  techniques in the Pharmaceutical, Foods and
  Paints Industries, as well as Water Laboratories

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APPNOTE - Inorganic

• SAMPLE River Water for PHOSPHATE
• PREPARATION Digest w/ 10 HCl, add NH4MoO4
  Hydrazine reagent, boil till Blue color develops
  measure at 810nm.
• ANALYSIS CE-2041 or VIS-100 w/ USEPA 2-pt
  Calibration

RESULTS mg/L or PPM in Sample
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APPNOTE - Inorganic

• DATE Total-PO4 ortho-PO4
• 3-10-00 69 PPM 51 PPM
• 3-24-00 77 PPM 50 PPM
• 4-08-00 94 PPM 83 PPM
• 4-22-00 118 PPM 72 PPM
• 5-10-00 85 PPM 78 PPM
• ANION assays by Colorimetry are the ACCEPTED
  Standard World-Wide much better (and less
  expensive) than Ion-Chromatography

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APPNOTE - Cosmetic

• SAMPLE Color-Dyes for Hair Shampoos
• PREPARATION Make 15 and 1200 dilution of Dyes
  to compare to Samples
• ANALYSIS CE-2041 or -3041 Scanning
  Spectrophotometer,
• 1cm. Glass Cuvette

RESULT Red / Green / Blue based on Color
Standards
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APPNOTE - Nutritional

• SAMPLE Cereals and Grains (preserved)
• PREPARATION Hydrolyze 2 grams w/ 5 HCl,
  extract w/ Hexane for 110 dilution
• ANALYSIS CE-2041 or CE-3041 Scanning
• Spectrophotometer, scan range 200-450nm. For BHT,
  BHA and TBHQ Anti-Oxidants. Sample solutions run
  in 10mm. Quartz Cells

RESULT Parts-Per-Million TOTAL Anti-Oxidants
based on USFDA Standard Procedure
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APPNOTE - Nutritional

• Sample BHA BHT TBHQ
• Wavelength 410nm. 360nm. 285nm.
• Frosted Flakes 35 PPM 27 PPM 11 PPM
• Raisin Bran 16 PPM 41 PPM lt 2 PPM
• Rice Krispies 23 PPM 11 PPM 4.5 PPM
• Scanning SPECTROPHOTOMETRY is an ideal
• method to evaluate YOUR OWN products, or material
  from the Competition

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Other SPECTRO Analyzers from BUCK

• BUCK Scientific also offers a series of
  inexpensive, high-performing and reliable
  Analytical Spectroscopy equipment for special
  Applications, Educational and Dedicated Testing
  procedures

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FLUORESCENCE SPECTROSCOPY (FLUOR)
Theory Principle of OPERATION

• FLUORESCENCE is a form of Molecular EMISSION
  Spectroscopy where a Sample in a special Cuvette
  is exposed to invisible, high-Energy UV
  radiation, and certain parts of the Organic
  Molecule will give off or EMIT lower-Energy
  VISIBLE Light radiation.
• Very specific selective test for Drugs
  Vitamins, Alkaloids Toxins

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BUCK Filter Fluorometer

• The advantage of HIGH Sensitivity for most
  compatible materials makes Fluorescence a
  valuable analytical tool for Forensic Drug
  applications

Intense BLUE Fluorescence of QUININE in a Drug
Formulation
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BUCK Filter Fluorometer

• The availability of a wide assortment of
  Excitation and Emission Filters makes this
  portable FL-380 instrument very flexibility for a
  very low cost ideal for Academic Teaching
  Industrial QA/QC work

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Visible COLORIMETRY (COLOR)
Theory Principle of OPERATION

• This is for use in Academic Teaching classes and
  for dedicated COLORIMETRIC Analyses for materials
  such as ANIONS (SO4, NO2, NO3, PO4, etc) and
  Visible Colors of compound such as Pigments,
  Chlorophyll and Dyes
• Covers the range from 320 - 950nm.

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BUCK Colorimeter

• The VIS-100 is a small, durable, self-calibrating
  unit that provides MEDIUM resolution _at_ 8nm.
  Bandpass, automatic 1-point Calibration Data
  Outputs for a very low price