13C APE

1
Supplemental data Figure 1
A
B
C
13C APE
19F/12C
1.1
1.500
0.8
1.125
0.5
0.750
0.2
0.375
-0.1
0
D
E
19F/12C
0.100
0.075
0.050
0.025
0
Supplemental data figure 1. NanoSIMS images of
13C-labeled V. cholerae cells after
fluorine-labeling by EL-FISH. A-C. Hybridization
with general Bacteria probe EUB338-I and tyramide
signal amplification (TSA) with tyramide 517F.
DE. Negative control. Hybridization with
background control probe NON338 and TSA with
tyramide 517F. A Secondary electron image
corresponding to panel B and C. B The
distribution of 13C enrichment. C The abundance
of 19F relative to 12C after hybridization with
probe EUB338-I. D Secondary electron image
corresponding to panel E. E The abundance of 19F
relative to 12C after hybridization with probe
NON338. Color bars indicate the relative
isotopic/elemental enrichment/abundance in the
image. APE atom percent enrichment.
2
Supplemental data Figure 2
A
B
C
19F/12C
0.10
0.07
0.05
0.02
10µm
0.00
D
E
F
19F/12C
0.10
0.07
0.05
0.02
10µm
0.00
Supplemental data figure 2. Probe mismatch
controls. Fluorescence and NanoSIMS images of
Bacillus subtilis (A-C) and Pseudomonas
aeruginosa (D-F) cells after EL-FISH. AD.
Positive control. Fluorescence images after
hybridization with general Bacteria probe
EUB338-I and tyramide signal amplification (TSA)
with tyramide 517F. BE. Secondary electron
images. CF. Mismatch control. Relative abundance
of 19F-fluorine to 12C-carbon after hybridization
with probe CF319a and TSA with tyramide 517F.
Color bars indicate the relative elemental
abundance in the image.