Cellular Biomechanics Kaitak Wan Asst Prof Mechanical Engineering UMR

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Cellular BiomechanicsKai-tak Wan (Asst
Prof)Mechanical Engineering UMR

• Mechanical characterization of
• Single cells mouse egg / embryo, stem cells
• Artificial cells liposomes, drug delivery
  capsules
• using AFM, MEMs, and micro-pipette.
• Adhesion and detachment of cells from glass
  substrate by osmosis

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Single Cell Characterization

• Measurements of
• Flexural rigidity
• Extensional modulus
• Residual stress
• Turgor pressure
• Membrane thickness
• Rubber elasticity
• Viscoelasticity
• Adhesion

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Indentation of a Mouse Embryo (In-Vitro
Fertilization)
Y Sun, KT Wan, J Bischof, K Roberts and BJ
Nelson, Mouse Zona Pellucida elastic property
characterization and modelling IEEE Transactions
on NanoBioScience, vol 2, iss 4, pp 279-286,
2003.
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Cell detachment as a result of osmosis
Adhesion of lipsome onto glass substrate in
isotonic solution large contact area
Detachment due to osmosis in dilute
solution Point contact with substrate