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BIOLOGY ADVENTURES
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PRESENTS
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v
Sea Urchin Embryology
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Discover and Explore the secrets of Animal
Reproduction
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Section 1
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Field Trip by Pamela Miller
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Lecture by Dr. David Epel
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Experiment by Dr. Jason Hodin
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Section 2
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My experiment
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WELCOME!
Dr. Epel - Hopkins Marine Station Stanford
University
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BIOLOGY ADVENTURES
Sea Urchin Embryology
The Experience at Hopkins Marine Station
My Experiment
Visit the researchers at the Hopkins Marine
Station and discover the reproduction and life of
the Sea Urchin
Do the experiment, manage the variables and
report the observations
For the best experience with this site, click
down the left column first then the right.
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The Experience at Hopkins Marine Station
What is it ?
What is it ?
What is it ?
What is it ?
What it is
How it works?
How it works?
How it works?
How it works?
How it works
The late Daniel Mazia was a biological research
scientist who first studied cell embryology using
Sea Urchins. Scientists at Marine Hopkins Station
of Stanford University work on similar cell
biology experiments. In this experiment, the eggs
of sea urchins are externally fertilized with sea
urchin sperms and the various developmental
stages of the fertilization process are observed
with a microscope.
Classes
Classes
Classes
Field Trip
Field Trip
Biology Lecture
Biology Lecture
Biology Lecture
Lecture
Lecture
Experiment
Urchins Life
Urchins Life
Urchins Life
Experiment
The experiment
The experiment
The experiment
Class Report
Test Results
About Us
Class Report
Class Report
Class Report
About Researchers
About Researchers
About Researchers
About Researchers
Hopkins Marine Station
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The Experience at Hopkins Marine Station
Teacher Pam Miller will conduct the field trip,
Dr. David Epel the lecture, and Dr. Jason Hodin
the experiment, so in this section, you will
learn about the history of fertilization, the
various forms of life, and the life of sea
urchins. In the experiment, you will learn about
the different stages of fertilization such as
mitosis, nuclear migration, zygota, and
gastrulation. You will receive questions at the
end of every topic to reinforce your
understanding.
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My Experiment
My experiment is a virtual laboratory where you
will have an opportunity to perform the cell
embryology experiment using Sea Urchins. You can
perform the steps in the experiment and observe
the effects of variables on the developmental
stages of the fertilization process. You will
also be able to test your knowledge of the
experiment.
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My Experiment
My experiment allows you to test your knowledge
of cell biology. You can choose a topic, select a
hypothesis and design your own experiment. You
can then observe the effects of different
variables on the sperms, eggs, or cells. Finally,
you will compare your results with that of the
researchers to test the validity of your
hypothesis. You can also email the scientists at
the Marine Hopkins Station with your questions or
comments. After completing the experiment, you
can submit a lab report to your virtual
instructor.
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BIOLOGY ADVENTURES
Sea Urchin Embryology
The Experience at Hopkins Marine Station
My Experiment
What is it ?
What is it ?
What is it ?
What is it ?
What it is
What it is
pollution
How it works?
How it works?
How it works?
How it works?
How it works
How it works
Classes
Classes
Classes
Field Trip
My experiment
Field Trip
Biology Lecture
Biology Lecture
Biology Lecture
Lecture
Lecture
Experiment
Urchins Life
Urchins Life
Urchins Life
Experiment
The experiment
The experiment
The experiment
Class Report
Test Results
Test Results
About Us
Class Report
Class Report
Class Report
Discussion Board
FAQ
About Researchers
About Researchers
About Researchers
About Researchers
Hopkins Marine Station
Site Map
Growing up
Visit the researchers at the Hopkins Marine
Station and discover the reproduction and life of
the Sea Urchin
Do the experiment, manage the variables and
report the observations
For the best experience with this site, click
down the left column first then the right.
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The Experience at Hopkins Marine Station
Habitat
Species
Interdependence
Feeding
Reproducing
Teacher Pam Miller will show us the environment
and life of sea urchins in the Coast of
California (click on the video to see the entire
field trip without interruption, or click on each
chapter to see them selectively and to take a
quick test after each)
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The Experience at Hopkins Marine Station
Habitat
What is it ?
What is it ?
What is it ?
What is it ?
What it is
Species
How it works?
How it works?
How it works?
How it works?
How it works
Interdependence
Classes
Classes
Classes
Field Trip
Field Trip
Biology Lecture
Biology Lecture
Biology Lecture
Feeding
Lecture
Reproducing
Lecture
Experiment
Urchins Life
Urchins Life
Urchins Life
Experiment
The experiment
The experiment
The experiment
Class Report
Test Results
About Us
Class Report
Class Report
Class Report
About Researchers
About Researchers
About Researchers
About Researchers
Hopkins Marine Station
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The Experience at Hopkins Marine Station
Habitat
Species
Interdependence
Feeding
Reproducing
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The Experience at Hopkins Marine Station
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
How nudibranch reproduces? Male copulate on one
female Several male copulate one female
Hermaphrodite copulate hermaphrodite Three or
more hermaphrodites copulating together
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The Experience at Hopkins Marine Station
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
How nudibranch reproduce? Male copulate on one
female Several male copulate one female
Hermaphrodite copulate hermaphrodite Three or
more hermaphrodites copulating together
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The Experience at Hopkins Marine Station
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
How nudibranch reproduce? Male copulate on one
female Several male copulate one female
Hermaphrodite copulate hermaphrodite Three or
more hermaphrodites copulating together
Very Good. You answered correctly. Now try a
slightly more difficult question!
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The Experience at Hopkins Marine Station
Biology
Developmental Biology
Embryology
Embryo
Sea Urchin Embryology
Professor Epel will explain the origins and
meaning of the Embryology
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The Experience at Hopkins Marine Station
Biology
Biology
Developmental Biology
Developmental Biology
Embryology
Embryology
Embryo
Embryo
Sea Urchin Embryology
Sea Urchin Embryology
Biology is the study, or science, of life. It is
concerned with the characteristics and behaviors
of organisms, how species and individuals come
into existence, and the interactions they have
with each other and with the environment.
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The Experience at Hopkins Marine Station
Biology
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
Developmental Biology
How many domains of living things are
there? one two three four
Embryology
Embryo
Sea Urchin Embryology
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The Experience at Hopkins Marine Station
Steps
1. Gametes
2. Fertilization
3. Cell Division
4. Growing up
The experiment comprises of four sections. Pay
attention to how Dr. Jason Hodin first forms a
hypothesis, collects data, analyzes data and
draws a conclusion. At the end of each section,
you will get a chance to test your understanding
of the material.
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
In this section, we will watch Dr. Jason Hodin
first spawning and collecting sea urchin gametes.
We will observe these gametes closely with the
use of a microscope, playing close attention to
attributes such as their size, proportions,
color, movement and more. Then we will compare
sea urchin gametes to that of humans. We will
end experiment part 1 with a short test.
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
Gametes, also known as sex cells, are the
specialized germ cells that come together during
fertilization (conception) in organisms that
reproduce sexually. In those species that produce
two morphologically distinct types of gametes,
and in which a particular individual produces
only one type, "females" of the species produce
the larger gamete called an ovum (or egg) and
"males" produce the smaller gamete termed a
spermatozoon (or sperm cell).
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
Here we pick a male and a female sea urchin and
inject them with KCl
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The Experience at Hopkins Marine Station
male (sperm) female (eggs)
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
Now we extract gametes from male and female sea
urchins
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
We collect male and female gametes
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
Using a microscope, we observe eggs
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
We pay attention to attributes such as color and
size of eggs
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
We can observe sea urchin sperm
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
And human sperm
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The Experience at Hopkins Marine Station
Steps
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
And we compare sea urchin sperm and human sperm
Nowwhat have you learned?
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The Experience at Hopkins Marine Station
Steps
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
Whats the color of an unfertilized urchin
egg? Yellow White Purple Red
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
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The Experience at Hopkins Marine Station
Steps
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
Whats the color of an unfertilized urchin
egg? Yellow White Purple Red
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
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The Experience at Hopkins Marine Station
Steps
TEST YOURSELF!
Q1 Q2 Q3 Q4 Q5 Q6 Q7
Step 1 Collecting Sperms and Ovaries, mix, and
put them on the slide
2. Fertilization
1. Gametes
Whats the color of an unfertilized urchin
egg? Yellow White Purple Red
3. Cell Division
2. Fertilization
4. Growing up
3. Cell Division
4. Growing up
Sorry. Wrong Answer. Click on the above video to
repeat the part on gamete color.
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The Experience at Hopkins Marine Station
Performance 80 Lecture 90 Field Trip 50
Experiment Recommendation It appears that you
can strengthen your knowledge on the experiment
section. We recommend you repeat the development
section again.
Answers/Questions 28/35 33/35 35/35
Grades 3.5 GPA 3.8 GPA 2.0 GPA
Overall Grade 2.8 GPA
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The Experience at Hopkins Marine Station
Research
Dr. Jason Hodin
Dr. David Epel
Pamela Miller
CV
CV
CV
Media Development
Sara Kalantari
Deepak Kumar
Wuping Lu
Dr. Decker Walker
CV
CV
CV
CV
CV
CV
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The Experience at Hopkins Marine Station
Hopkins Marine Station, located on the Monterey
Peninsula in California, is a marine biology
research and educational facility that operates
as a branch of Stanford University's Department
of Biological Sciences. Founded in 1892, Hopkins
was the first marine laboratory to be established
on the American Pacific coast. Since that time,
many notable scientists, as well as undergraduate
and graduate students have come to the Station to
study and work towards a better understanding of
the ocean and its species.
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My Experiment
Design your experiment

1. Hypothesize
2. Design your experiment use the UTOS guidelines
3. Compare your experiment with the Expert version
4. Analyze the expert data
5. Apply the knowledge to the hypothesis
6. Reach a conclusion Validate or refute the
   hypothesis

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My Experiment
Unit
Treatment
Observation
Situation
Design your experiment following these
guidelines. Hypothesis A a proposed
explanation for a phenomenon. Unit Choose
your subject of study. Treatment Choose the
variables you want to manipulate. Observation
Choose what you will look for. Situation
Choose your environment/situation for the above.
Design your experiment (UTOS) - guidelines
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My Experiment
Hypothesize Choose a hypothesis you would like
to test.

1. Sea urchin fertilization is affected by the
   temperature of its environment.
2. UV causes damage to genetic and protein material
   in sea urchin embryo
3. Is it the ions in the seawater or the osmotic
   pressure that is important for fertilization and
   normal development?
4. The wave action of the ocean affect sea urchin
   embryos?

Your Hypothesis UV causes damage to genetic and
protein material in sea urchin embryo
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My Experiment
Unit
Treatment
Observation
Situation
What are you going to study? Choose from below.
Protein Molecule
Sea Urchin Eggs
Sea Urchin Sperm
Sea Urchin Embryo
Your Unit Sea Urchin embryo
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My Experiment
Unit
Treatment
Observation
Situation
Choose the variable you want to change in your
experiment.
CO2 Mg Fe Ca
Sunlight
Formula of water
Temperature
Artificial UV light
Your Treatment Sunlight
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My Experiment
Unit
Treatment
Observation
Situation
Choose what will you look for in the experiment.
Change in shape of the gametes
Decrease in development
Lack of Movement
Change in Color
Your Observation Change in shape of the gametes
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My Experiment
Unit
Treatment
Observation
Situation
Choose your environment/situation for the above.
Beaker Outdoors
Ocean
Petri dish in Lab
Your Situation Ocean
Submit
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My Experiment
Unit
Treatment
Observation
Situation
Summary of your experiment
Hypothesis UV causes damage to genetic and
protein material in sea urchin embryo. Unit
Sea Urchin embryo Treatment Sunlight Observation
Change in shape of the gametes Situation
Ocean
Next
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My Experiment
Your experiment
Scientists experiment
Hypothesis UV causes damage to genetic and
protein material in sea urchin embryo. Unit
Sea Urchin embryo Treatment Sunlight Observation
Change in shape of the gametes Situation
Ocean
Hypothesis UV causes damage to genetic and
protein material in sea urchin embryo. Unit
Sea Urchin embryo Treatment Artificial
Ultraviolet Observation Decrease in
development, lack of movement Situation Petri
dish in Lab

• .

x
x
x
Compare your experiment with experiment the
scientist actually tested.
Next
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My Experiment
Analyze the data from the scientists experiment.
Graph 2
Graph1

1. What kinds of different lights can you identify
   that may have effects to sea urchin embryos.
2. What does the graph 2 indicate? Why did this kind
   of data come up?

Next
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My Experiment
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My Experiment
Professor Epel will explain what they found from
this data.
We found that not all UV lights are harmful to
sea urchin embryos. There is some light called
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My Experiment

1. What do you think happened to the sea urchin
   embryos from this picture?
2. Can you identify where there is a damage?

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My Experiment
Record of all my experiment results
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My Experiment
Discussion Board
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My Experiment
What is it ?
What is it ?
What is it ?
What is it ?
What it is
How it works?
How it works?
How it works?
How it works?
How it works
Classes
Classes
Classes
Field Trip
Field Trip
Biology Lecture
Biology Lecture
Biology Lecture
Lecture
Lecture
Experiment
Urchins Life
Urchins Life
Urchins Life
Experiment
The experiment
The experiment
The experiment
Class Report
Test Results
About Us
Class Report
Class Report
Class Report
About Researchers
About Researchers
About Researchers
About Researchers
Hopkins Marine Station
Experiment
My Experiment
Field Trip
Lecture
62
My Experiment
FAQ
63
My Experiment
Site Map
64
Active Page
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BIOLOGY ADVENTURES
Sea Urchin Embryology
The Experience at Hopkins Marine Station
My Experiment
What is it ?
What is it ?
What is it ?
What is it ?
What it is
What it is
pollution
How it works?
How it works?
How it works?
How it works?
How it works
How it works
Classes
Classes
Classes
Field Trip
My experiment
Biology Lecture
Biology Lecture
Biology Lecture
Field Trip
Lecture
Lecture
Experiment
Urchins Life
Urchins Life
Urchins Life
Experiment
The experiment
The experiment
The experiment
Test Results
Class Report
Test Results
About Us
Discussion Board
Class Report
Class Report
Class Report
FAQ
About Researchers
About Researchers
About Researchers
About Researchers
Hopkins Marine Station
Site Map
Growing up
Visit the researchers at the Hopkins Marine
Station and discover the reproduction and life of
the Sea Urchin
Do the experiment, manage the variables and
report the observations
For a better understanding of the site click in
order every menu bar from top to down, first the
left column, then the right column