NADH is not transported DIRECTLY into the matrix

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NADH is not transported DIRECTLY into the matrix
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For 2H 2NADH O2 ? 2NAD H2O
10 H pumped/2 e- or 10 H pumped /NADH
For 2FADH2 O2 ? 2FAD 2H2O
6 H pumped/2 e- or 6 H pumped /FADH2
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see Table 12-1, p373
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In the e- transport system e- are transferred
from species with lower standard reduction
potential to those with higher standard reduction
potential
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Complex I transports 4H/2e- NADH H Q
4H(in) ? NAD QH2 4H(out)
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How are e- transport and H transport coupled? -
protein conformational change - redox loop
mechanism
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(IMS)
Redox loop mechanism CoQ is reduced (and
therby picks up 2 H) on the matrix side of IMM.
The reduced CoQH2 then moves to the IMS side of
the IMM where it is oxidized (and thereby gives
up 2H).
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Complex III transports 4H/2e- 2cytc(ox) QH2
2H(in) ? 2cytc(red) Q 4H(out)
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Intermembrane space
membrane
matrix
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CoQ-Cytc oxidoreductase (Complex III)
Redox centers in Complex III
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Complex IV transports 2H/2e- 2cytc(red) ½O2
4H(in) ? 2cytc(ox) H2O 2H(out)
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Cytc oxidase (Complex IV)
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See Fig 12-18
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oxidative phosphorylation
oxidative refers to electron transport, which
oxidizes upstream cofactors such as NADH and
FADH2 (which, in turn, oxidize nutrients).
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Intermembrane space (lower pH) Matrix (higher
pH)
ATP synthase (F1F0 ATPase)
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X-ray analysis shows 3 different conformations
for the headpiece tight loose open
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Aerobic
Anaerobic
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e- transport and oxidative phosphorylation
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2
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3 ATP / NADH or Roughly 3.3 H / ATP
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See problems 14-18 in Chapter 12
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Sample Problem
Draw O2 uptake curve that results when the
following are added in the indicated order to a
suspension of mitochondria a) glucose b) ADP
Pi c) citrate d) oligomycin e) succinate f)
dinitrophenol g) rotenone h) cyanide