Chesapeake Bay Phytoplankton Index of Biotic Integrity

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Chesapeake Bay Phytoplankton Index of Biotic
Integrity

• Prepared by
• Richard V. Lacouture ANSERC
• Dr. Claire Buchanan ICPRB
• Dr. Harold Marshall ODU
• Jacqueline Johnson ICPRB

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History of Phyto IBI Development

• 1998-2001 water quality reference conditions
  established by means of MANOVA and canonical
  discriminant analysis and best phytoplankton
  indicators detected by stepwise discriminant
  analysis.
• 2001-2002 water quality binning technique using
  DIN, PO4 and secchi depth classifies each data
  point and associated phytoplankton parameters
  analyzed for discriminatory strength by looking
  at the spread and overlap between 25th and 75th
  percentiles amongst the different water quality
  categories.
• 2002-2003 expanded the array of phytoplankton
  parameters used in association with the water
  quality binning technique.

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Comparison of Results

• ODU Technique
• Salinity Regime Correctly
• Classified
• TF 64
• OH 60
• MH 51
• PH 68
• Total 59
  

• Binning Technique
• Salinity Regime Correctly
• Classified
• TF 45
• OH 46
• MH 48
• PH 60
• Total 49

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Refinements to the Binning Technique

• Parameters in New Technique
• Above pycnocline chlorophyll a
• Above pycnocline pheophytin
• Diatom carbon
• Dinoflagellate carbon
• Cyanophyte carbon
• Total phytoplankton carbon
• Chlorophyte carbon
• Cryptomonad density
• Diatom density
• Microcystis aeruginosa density
• Prorocentrum minimum density
• Mean cell size
• CarbonChlorophyll Ratio
• TSS
• DOC

• Parameters in Old
  
  
  Technique
• Above pycnocline chlorophyll a
• Primary productivity
• Diatom carbon
• Dinoflagellate carbon
• Cyanophyte carbon
• Total phytoplankton carbon

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Comparison of Binning Technique Results

• Old Technique Summer
• Salinity Regime Correctly
• Classified
• TF 64
• OH 46
• MH 48
• PH 64
• Total 53

• New Technique Summer
• Salinity Regime Correctly
• Classified
• TF 89
• OH 72
• MH 71
• PH 73
• Total 75

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Next (and Final) Steps

• May 1 - Meet with Dr. Buchanan to analyze spring
  results and complete spring parameter selection
  and threshold development
• May 03 - Run verification tests on spring data
  to test for predictability of IBI vs water
  quality binning categories
• May 03 - Write up results as part of MDDNR
  Level I report
• July 03 - Submit manuscript describing
  Chesapeake Bay Phytoplankton IBI and its
  application Baywide