Welcome to Microbiology Lab

1
Welcome to Microbiology Lab

• Tuesday Section F, Room 124
• Wednesday Section C, Room 125

2
Todays Lab

• Enrollment-Phantom Students, Roll Call
• Introductions
• Required Materials
• Laboratory Protocol and Safety
• Begin Environmental Isolate

3
Enrollment

• Roll call
• Seating Chart
• Phantom students
• Administrative Drops

• Check your Schedules!
• Tuesday Section F, Room 124
• Section D is next door in Room 125
• Wednesday Section C, Room 124
• Section H is next door in Room 125

4
Introductions

• Lab manager Linda Fitchett
• Linda.Fitchett_at_nau.edu
• Phone [phone]
• Office 114
• TAA

5
Introductions Me

• TA Breanne Espen
• Email bjk35_at_nau.edu
• Office 107

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Websites

• Micro Homepage
• www4.nau.edu/biology/bio205
• My website
• pine.ucc.nau.edu/bjk35/

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Required Materials

• Items you must bring to Lab every week
• Lab manual
• Lab coat
• Photo atlas
• Sharpie (fine tip)
• Text Book
• Check items in lab drawer

8
Laboratory Protocol and Safety

• Overview of Lab Manual
• Experiments
• Space for notes
• Mini Writing Assignments

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Laboratory Protocol and Safety

• Lab Rules
• Page 7 (or vii) in the lab manual
• Write your name at the top, sign at the bottom,
  initial by each rule

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Laboratory Protocol and Safety

• Disposal of items that will be used in the lab
• Trash
• All gloves in contaminated waste
• Plate Bin
• Trash
• Nalgene buckets
• Contaminated glass, uncontaminated glass

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Laboratory Protocol and Safety

• Tour of the Lab
• Shower
• Eye wash (bottles)
• Fire Extinguisher
• Safety Quiz

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Environmental Isolate

• This is a semester long project!

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Medium

• Medium In microbiology, any liquid or solid
  preparation made specifically for the growth,
  storage, or transport of microorganisms or other
  types of cells.

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Medium properties

• Once set, the agar is stable at temperatures up
  to 65C or higher.
• Few organisms are able to degrade agar although
  it does dehydrate.
• Agar is a transparent gelatin-like substance
  that is formed when a mixture of agar and water
  is heated to gt100C and then cooled gelling
  occurs at 40-45C.

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Medium properties

• Growth media is prepared by adding nutrients,
  selective agents, etc. to agar usually before
  autoclaving.
• Agar is produced by red-brown algae and is widely
  used as a base for many kinds of solid and
  semi-solid microbiological medium.
• Rhodophyceae algae (red-brown algae) is a primary
  source of agar.

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Medium used today

• -Trypticase Soy Agar (TSA)
• a general purpose medium
• agar-based containing a pancreatic digest of
  casein (trypticase, tryptone), a papain digest of
  soybean meal (phytone), and NaCl (0.5 w/v).
• -Trypticase Soy Broth (TSB)
• is the same general purpose medium without agar
  base.

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Procedure

• Supplies
• 3 plates (check for unwanted growth)
• Test tube with 3 cotton swabs
• Sharpies
• Demonstration
• How to label a plate
• How to remove cotton swabs
• How to streak a plate

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How to Label Agar Plates

• Always label the bottom, side of the plate!
• Label every plate with
• Name
• Date
• Lab time
• Instructor
• Experiment

• Label Plate agar side up

Name, section, date, lab time, instructor
Plate, agar side up
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How to Label Agar Plates

• Plate 1 Control
• Control is a reference point in a controlled
  experiment in which a set of conditions does not
  vary
• Plates 2 and 3 will be labeled A and B
• record their sources (extra space for notes is
  provided in your manual)

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Swabbing the Plate

• Demonstration
• How to remove swabs
• How to swab the plate
• 
  Agar Plate

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Environmental sources

• Absolutely no human sources!
• Ex. door knobs. bathrooms, cars, drinking
  fountains, etc.
• Animals and their feces are also unacceptable
• Acceptable Sources
• Ex. trees, rocks, grass
• Use your imagination!

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Environmental Isolate

• Plates will incubate for one week
• Next week
• Begin purification streaks
• Begin working with a partner

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Syllabus

• Mini-writing assignments
• 4 mwa (5 points each) 20 points
• Lab reports
• 2 reports (40 points each) 80 points
• Environmental Isolate 80 points
• Work Sheets
• 4 worksheets (10 points each) 40 points
• Quizzes
• 7 quizzes (5 points each) 1 dropped 30 points

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Syllabus

• Gram stain unknown quiz
• All or nothing quiz
• 20 points
• Technique
• 15 points
• Sterile technique, staining, microscope care,
  etc.
• Professionalism
• 15 points
• Wearing lab coat, arriving on time, paying
  attention, etc.

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Syllabus

• Late work will NOT be accepted. All work is due
  at the beginning of the lab period.
• You will have two chances on the first two lab
  reports. After the first is graded, ALL
  corrections must be made.
• If you do not follow the directions of the Lab
  reports they will be returned and no further
  submissions will be allowed.
• You will only have one chance on the
  Environmental Isolate report.

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Syllabus

• Attendance is mandatory!
• Missed quizzes can not be made up
• If you missed an experiment that you must write a
  report on, you will automatically miss 10 points
  off of the report.
• Because the labs are full, you will be allowed to
  attend another section only under extraordinary
  circumstances that you have discussed with the TA
  beforehand!

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Syllabus

• Plagiarism
• Plagiarism is NOT tolerated!
• Webster's Tenth New Collegiate Dictionary defines
  the word plagiarize as " to steal and pass off
  (the ideas or words of another) as one's own use
  (a created production) without crediting the
  source vi to commit literary theft present
  as new and original an idea or product derived
  from an existing source."

• If you plagiarize someones work, you could
  receive an F in the CLASS, not just the lab!
• Experiments are often in groups, and you are
  encouraged to discuss results, methods, etc. with
  your peers. However, collaboration STOPS when the
  report is written!

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Syllabus

• Schedule of Laboratory and Reading Assignments
• The dates of assignments and experiments are in
  your syllabus
• I will try to remind of the due dates, but it is
  YOUR responsibility to check the schedule every
  week!

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Next Week

• Assignments
• Mini-writing assignment 1 due!
• Pre-lab 1 Due, dont copy example!
• Exercise 1
• Microscopy
• Continue Environmental Isolate