Welcome to BIO 205

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Welcome to BIO 205

• GTA Robert Martz
• Office 121 / 112
• 928-523-0553
• rjm56_at_dana.ucc.nau.edu

BIO205 Sec. H 3945 Tuesday 600-900PM TAA
Sarah Medoff BIO205 Sec. C 3267 Thursday
800-1100AM TAA
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Objectives

• Review Safety
• Review Lab Rules
• Review Lab
• Visit Office
• Break
• Safety Quiz
• Environmental Unknown

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Lab Safety

• Lab Coats
• Dress code
• Required materials
• Safety glasses and gloves
• Glass, contaminated material, and trash
• Cleaning

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Safety Quiz

• 1. When coming in to check cultures, you DO NOT
  have to wear your lab coat.
• TRUE FALSE
• You must ALWAYS wear your lab coat when in
  the lab.
• 2. Eating, drinking and applying lip-gloss while
  in the laboratory IS PERMITTED.
• TRUE FALSE
• Eating, drinking, and applying lip-gloss is
  NEVER permitted in the laboratory.
• 3. If your microscope is NOT in good working
  condition, you should try and fix it yourself.
• TRUE FALSE
• Bring microscope problems to the attention
  of your TA.

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Safety Quiz

• 4. You should assume that the inoculating
  loop/needle is contaminated until it is
  sterilized in the flame.
• TRUE FALSE
• 5. If an accident occurs where culture is
  spilled, you should clean it up immediately.
• TRUE FALSE
• Notify TA immediately of any culture spills.
• 6. When using culture tubes, it is acceptable to
  place them in the student bins with the stains
• TRUE FALSE
• ALWAYS place culture tubes in racks.

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Safety Quiz

• 7. Under certain circumstances, students may
  remove cultures from the laboratory.
• TRUE FALSE
• NEVER remove cultures from the laboratory.
• 8. You must wash your hands with soap and water
  upon arriving and before leaving the laboratory.
• TRUE FALSE
• 9. You must wash you work bench with Osyl and a
  damp sponge upon arriving and before leaving the
  laboratory.
• TRUE FALSE

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Safety Quiz

• 10. It is NOT necessary to wear safety glasses
  when working with bacterial cultures.
• TRUE FALSE
• You are to wear safety glasses whenever
  you are working with bacterial cultures.
• 11. Gloves MUST be worn when working with stains
  and reagents that may be toxic to the skin.
• TRUE FALSE
• 12. If you are pregnant, or otherwise
  immunocompromised (i.e. HIV/AIDS, chemotherapy
  treatment, etc.), you may be at greater risk than
  otherwise.
• TRUE FALSE

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Safety Quiz

• 13. Each student is held responsible for the
  equipment that is either assigned to or used by
  them.
• TRUE FALSE
• 14. If a culture tube is dropped or broken, the
  student should clean it up immediately and then
  notify the TA.
• TRUE FALSE
• Immediately notify your TA if culture
  tubes are spilled, dropped, or broken.
• 15. Broken glass, such as slides or cover slips,
  should be disposed of into a waste basket or the
  nalgene buckets provided on the lab bench.
• TRUE FALSE
• Slides and cover slips are ALWAYS placed
  in slide trays at the ends of the benches.

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Safety Quiz

• 16. Open wounds should be covered before coming
  into the lab.
• TRUE FALSE
• 17. Mouth pipetting is allowed only if the TA is
  assisting you.
• TRUE FALSE
• You should NEVER mouth pipette.
• 18. Your lab coat should be laundered and
  bleached periodically during the semester.
• TRUE FALSE

10
Safety Quiz

• 19. Open-toed shoes are permitted in the
  laboratory during summer sessions only.
• TRUE FALSE
• Open-toed shoes are NEVER permitted in
  the laboratory.
• 20. Failure to follow directions from the
  laboratory instructor may result in dismissal
  from the class (both lecture and lab).
• TRUE FALSE

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Begin Environmental IsolatePart 1

• This is a semester long project
• This means extra hours working outside of
  scheduled class meeting times.
• -Show an example of a properly labeled plate and
  briefly discuss media and its properties.
• -Medium In microbiology, any liquid or solid
  preparation made specifically for the growth,
  storage, or transport of microorganisms or other
  types of cells.

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Trypticase Soy Agar(the medium used today)

• -Trypticase Soy Agar (TSA) a general purpose
  medium, agar-based containing a pancreatic digest
  of casein (trypticase, tryptone), a papain digest
  of soybean meal (phytone), and NaCl (0.5 w/v).
• -Trypticase Soy Broth (TSB) is the same general
  purpose medium without agar base.

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Properties of TSA

• Once set, the agar is stable at temperatures up
  to 65C or higher.
• Few organisms are able to degrade agar although
  it does dehydrate.
• Agar is a transparent gelatine-like substance
  that is formed when a mixture of agar and water
  is heated to gt100C and then cooled gelling
  occurs at 40-45C.

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Environmental Isolate

• Obtain Sterile Cotton Swabs and three TSA plates
• One plate is a control, the remaining two plates
  will be used for your organisms.
• As a group, we will go outside, swab, and streak
  material onto the TSA plates as demonstrated in
  class.
• Remember, no material that you suspect would have
  been in contact with a human source or any other
  protein.

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Questions and Clean up