Bispecific Antibody Overview

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Bispecific Antibody Overview
Bispecific Antibody (BsAb) are antibodies that
can simultaneously bind two separate and unique
antigens (or different epitopes of the same
antigen). The primary application of BsAb has
been to redirect cytotoxic immune effector cells
for the enhanced killing of tumor cells by
antibody-dependent cell-mediated cytotoxicity
(ADCC) and other cytotoxic mechanisms mediated by
the effector cells.
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Bispecific Antibody Structure and Formats
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Schematic of IgG Antibody Production Using
Transient Expression
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IgG Antibody Production Steps
IgG Antibody Gene Synthesis and Subcloning After
customer's approval, target DNA sequences are
designed and synthesized. The complete sequences
are subcloned into a proprietary vector for
expression. IgG Antibody Cell Culture and
Transient Transfection Cells are grown in
serum-free media. Recombinant plasmids encoding H
and L chains are transiently co-transfected to
suspension cell cultures. Cell culture
supernatant is collected on day-5,
post-transfection for SDS-PAGE analysis, in order
to evaluate expression levels. IgG Antibody
Purification and Analysis Purification is
carried out using protein A column. Eluted
fractions are analyzed by SDS-PAGE and Western
blot and pooled and buffer exchanged to the final
storage buffer. Deliverables Synthetic genes in
pUC57 vector, optimized gene sequence report and
purified antibody at listed amount and purity
were delivered to the client along with QC data
and comprehensive report.  
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Bispecific IgG Production
Purified Bispecific IgG Antibody SDS-PAGE (left)
and Western Blot of the same panel (right) under
reducing and non-reducing conditions
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