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Micropipette Calibration in UAE

• Presented by
• Arabian Calibration Dev. Rep. Co. LLC
• https//www.arabcal.com/

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Introduction

• All the laboratory equipment including the
  micropipette needs to be properly calibrated.
• The calibration limits the errors in volume
  discrepancies while enhancing the pipette
  efficiency for measurement and dispensing.
• Calibration ensures whether the equipment is
  dispensing the specified volumes properly and if
  not it needs to be fixed with proper medical lab
  calibration services.
• Core to pharmaceutical laboratory operations, the
  ISO 17025 certified pipette calibration services
  are in demand.

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Standard methods for pipette calibration

• Gravimetry
• Fluorometry
• Single-dye photometry
• Ratiometric photometry

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Gravimetry method for micropipette
calibration

• It involves a specialized balance (measurement up
  to 5-6 decimals) for weighing the volume of
  liquids.
• The balance gives a weight which then converted
  into mass followed by volume conversion using
  standard formulas methods.

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Fluorometry method for micropipette calibration

• This method is based on the measurement of the
  different wavelengths emitted by the liquid
  molecules when an ultraviolet light beam is shown
  on the sample liquid.
• On comparing the excitation wavelength emission
  wavelengths based on relative fluorescence
  levels, the precision result is drawn.

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Single-dye photometry method for micropipette
calibration

• A light beam is passed through the cuvette
  containing dye solution.
• Then, a photometer is used to measure how much
  quantity of light passes through it.
• Using the principle of proportionality that the
  amount of light absorbed is proportional to the
  amount of dye, the liquid volumes are determined.
  
• It is then followed by uncertainty analysis for
  error calculations.

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Ratiometric photometry method for
micropipette calibration

• The ratiometric method involves two standardized
  dyes for volume measurements.
• This helps in determining analyzing the
  absorbance ratios more accurately than individual
  absorbances of liquids as in single-dye
  photometric method.

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Reach us

• https//www.arabcal.com/
• Call Us- 971 6 5345747
• Fax [phone]
• Visit us - P.O. Box 24650,Industrial Area 13
  Near National Paints R/A,Sharjah, UAE.
• Contact Us - cs_at_arabcal.com