Blastocyst Culture

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Blastocyst Culture
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Blastocyst culture refers to growing the embryos
in the laboratory for two more days at which
point they are referred to as blastocyst embryos.
Blastocyst transfer simply means that the
blastocyst embryos are transferred to the womans
uterus on day 5 in exactly the same way that day
3 embryos .
Three important factors previously limited the
number of embryos that could survive to this
stage (development stage)
First, the "health" of any embryo will decided
its ability to continue to grow and divide.
Several eggs may have initially fertilized, but
only a few will progress to the four-cell stage
on day two in culture, fewer still to the
eight-cell stage on day three in culture, and
even fewer will develop into blastocysts. This
stage can be called as "survival of the fittest."
The second, limiting factor in preventing
continued growth of embryos in culture had been
the culture media Or blastocyst media.. These are
rich in life-sustaining nutrients that give the
embryos every chance to reach there full inherent
potential. As a result , embryos will now be
exposed to specific media that are designed to
meet the nutritional requirements of each stage
of development.
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Third, even with embryos of good growth potential
and optimal culture media, the experience and
expertise of the laboratory embryologists doing
this important work is critical to achieving good
blastocyst development.
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Advantages of blastocyst -
1) It makes a better selection of embryos
possible. Embryos which reach this stage offer a
better implantation capacity. It is for that
reason that we limit transfers to 1 blastocyst
(or maximum 2), reducing the risk of multiple
births.
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2) It allows a more physiological synchronisation
between the embryonic phase and the
responsiveness of the endometrium. 3) It
facilitates the correct supervision of embryo
development and the transfer of only the embryos
selected by Nature to reach an optimum state of
development, which in turn allows for a higher
implantation rate.
Disadvantage of blastocyst transfer -
4.However, if the culture environment is
suboptimal, delayed embryo development and even
embryonic arrest will occur at least in some
cases. Therefore, if the culture system and
quality control in an IVF lab are inconsistent,
good results will not be obtained. The only
disadvantage is lack of facilities and knowledge
to embryologists.
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