Stable Cell Line Construction for Protein Expression

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Stable Cell Line Construction for Protein
Expression
Profacgen
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When large amount of recombinant protein is
required, stable expression line construction is
recommended for reproducible and cost-effective
protein production. Construction of a stable cell
line usually takes 3 steps delivery of the
target gene into the host cell, screening of
stable expression clones, and stability test.
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Features High yield and reproducibility High
cell line stability One-stop service Service
fully customizable
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At Profacgen, we use lentiviral vector for
efficient gene delivery and to achieve high
integration rate. Limited dilution and
antibiotics screening are then applied to select
for high-yield, genetically homogenous stable
expression monoclones. When fluorescent protein
is fused to the target protein, we can also use
flow cytometry to screen for positive clones.
Profacgen also offers the generation of Tet-ON
inducible stable cell lines for inducible
expression of your target gene. Doxycycline can
then be used to induce the expression of your
target gene.
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To ensure integration of your target gene and
stable/inducible expression of the protein, we
can provide PCR, qRT-PCR, microscopy, flow
cytometry analysis and stability test according
to your specific requests.
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Profacgen provides stable cell line development
service both for research use and for industrial
production. We guarantee well-developed stable
cell lines with robust and reproducible
production of your target protein.
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Thanks for watching! Contact Profacgen Address
45-1 Ramsey Road Shirley, NY 11967,
USA Website https//www.profacgen.com