Title: ICSI in Hyderabad (1)
1ICSI in Hyderabad
www.kiranivfgenetic.com
2- ICSI in Hyderabad
- ICSI (Intra cytoplasmic sperm injection) is
an ART (assisted reproductive technology) used to
treat sperm related infertility problems. ICSI is
used to improve the fertilization phase of IVF
(in vitro fertilization) by injecting the single
sperm into mature egg. The fertilized egg is then
placed in a womans uterus/fallopian tube.
www.kiranivfgenetic.com
3- Importance
- Intra cytoplasmic sperm injection (ICSI) is used
to take care of severe male infertility, as when
little or absence of sperm are ejaculated in the
semen. Immature sperm composed from the testicles
are usually not able to move about and are more
likely to fertilize an egg during ICSI. - Some couples might choose to try ICSI after
frequent in vitro fertilization has been
ineffective. - ICSI is also used for many couples who are
planning to have genetic testing of the embryo to
check for certain inherent disorders. ICSI uses
only single sperm for each egg. So there will be
no chance the genetic test can be infected by
other sperm.
www.kiranivfgenetic.com
4www.kiranivfgenetic.com
5Sperm collectionBefore collecting the sperm,
male partner should be screened for possible
genetic problems that could pass on to the
offspring . If the sperm cannot be collected
through masturbation, they must be surgically
removed from the testicle through a small slit.
Ovulation and egg retrievalThe woman should be
injected with gonadotrophin or follicle
stimulating hormone (FSH) to inspire the ovaries
to generate multiple eggs. After 6 to 7 days, the
doctor should check the blood estrogen altitude
and use ultrasound to see whether eggs are
growing in the follicles. In the second week, the
dosage may change based on test outcome and
ultrasound. If follicles fully develop, the woman
is given a HCG (human chorionic gonadotrophin)
for the follicles to mature. The matured eggs
should be collected and later using needle
aspiration directed by ultrasound through the
pelvis to the ovaries.Sperm injection and
transferunder high power magnification, an egg
is held in place with a glass pipette. Another
pipette containing sperm is used to penetrate and
inject one sperm to the egg. After culturing in
the lab overnight, eggs are checked for signs of
fertilization. Two Blastocysts or four day 2/3
embryos are placed in the uterus using embryo
transfer catheter that is inserted through the
cervix.
www.kiranivfgenetic.com
6Thank You
www.kiranivfgenetic.com