King Lab Yearly Safety Review

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King Lab Yearly Safety Review

• by Cammie Haase-Pettingell
• for March 2005
• Disclaimer-This is Jonathan Kings lab in
  Biology Dept. yearly safety review and not to be
  substituted for an initial entry orientation.
  Any questions should be directed to Cammie 3-3545

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Points be covered

• Emergency Action Plan
• Eating
• Chemical
• Radioactive
• Biologicals, Blood Borne Pathogens, Infectious
  Agents
• High Voltage

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Responsibility

• PI
• Ensure that the lab is conducting in a safe manor
• This is by the PI or by a representative the
  safety rep.
• Safety Rep.
• To conduct incoming safety orientation
• Go to safety meetings
• Update the lab on new policy
• Individual
• To follow the above policy

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Basics

• No Eating in lab
• Can eat in
• Tea room
• Computer room ,
• hall and seminar room
• No smoking except outside
• No mouth pipeting
• Story

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Evacuation Plan
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Fire

• Close the lab doors
• Pull the fire red fire alarm
• Located by Stairwells , Elevators
• Leave by stairwells
• Get to a phone- dial x100 report fire
• Dont hang up till they have hung up
• Not responsible to fight a fire

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Unknown fire such as strange smell

• If in doubt call x 100
• Pull fire alarm
• Other scenario
• Look around
• Call John Fuccilo to ask he know anything
• Ex . melted stuff in autoclave

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Evacuation

• The equipment halls cant hear the alarm
• As exit ensure people know about the alarm
• If can - put experiments to rest- not required
  you safety is more important
• So firefighters
• ie do not stumble upon a active electrophoresis.
• Just in case not back for a while may not be
  ruined
• Evacuate by stairwell -all are fire stairwells
• Meet by the concrete post by bld 66
• even if you think I saw you Check in so we know
  no one is stuck in the building
• Then go off for what ever.
• Some one needs to go to the command post say we
  are all out
• by the door toward Main St. -

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Chemicals

• MSDS see handout
• Chemical safety web training
• http//mitehs.traincaster.com/
• Spill kits
• 68-340D behind door
• 68-322 under sink

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Hoods

• Simple Laminar Flow Hood
• Ensure the back slot in back is clear
• have items on lifts so it is not blocked
• Simple test for flow
• to tape Kimwipe
• If malfunctioning- inform fixit Zone -A
  http//web.mit.edu/facilities/www/
• Rooms air flow and temperature is dependant on
  the hood.

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Spills

• Chemical
• Radioactivity
• Biological
• General
• Clear Area
• Get on appropriate safety gear
• Determine the Scope
• Use the appropriated cleaner
• Dispose correctly
• Look at the Red flip chart by phone

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Chemical Spill

• Evacuate area- put sign up
• Done appropriate safety - lab coat, eye
  protection, appropriate gloves
• Determine the necessary sorbant,
• For acid base or solvent.
• Pour around the outside of the spill - mix in
  Hold breath
• I use the dust pan and broom sweep up.
• Wipe up residue
• Dispose of as waste

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Chemicals and Medical Emergency

• Do not block body showers
• Eye wash put injured eye down flush for 15 min.
• Eyewash or distilled wash with tubing
• Needs to be tempered water
• Cut and injury- should go to Medical
• can be documented and treated properly
• Someone collapses
• Ensure you are not putting you self in danger
• If can rise -call EMT X100
• EMT will be concern that they are not in danger
• try to figure what happened

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Remember

• Emergency number X100
• If working alone at night
• inform some one on the Hall
• Or call campus police 3-1212
• Safety is important to everyone.

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Chemical waste-

• Waste needs a red tag
• Must be closed when not in use.
• Most is in electrophoresis room
• Silver- silver stain
• MEOH-
• Gel drying fix. , Transfer solution
• Ethidium bromide -
• Liquid and tips
• Agarose in the dark room
• Once full date and should be picked up
  immediately by law 2 days
• If can call dont date till wedded in link on
  King links page
• http//web.mit.edu/environment/environmental/ehs_s
  ervices/ehs_areas/hazardous_waste/forms/chem_colle
  ction.html

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Sharps

• Two types
• Biological or not chemical waste-gray bin
• If it is sharp from a biological lab it can not
  be just thrown out.
• Chemical waste- with red tags
• At the Bench
• Need to have covers and be covered when not in
  use.
• Labeled

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Radiation ext. 3-2180

• We use 14C and 35S to label rapidly proteins
• Disposal designated by 1/2 life
• 20-120day 1/2 life -35 S- 88 day 1/2 life
• Over 120 day 1/2 life 14C 5730 yr 1/2 life
• Type- dry (paper gloves), hi level aqueous,
  sharps/epi.
• Radioactivity stocks
• is stored in the locked refrig and freezer
  68-346D
• Most experiment are preformed in the 68-346D.
• Geiger counters in each side of the lab.
• Pancake probes more sensitive
• Ensure the battery is off when done.

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Radioactive spill

• Clear the area- block off
• Ensure they you have lab coat, safety glasses,
  double gloves, boots?
• Monitor the area with Geiger counter
• Mark the area- where there are counts
• One does not inactivate the radioactive just
  clean off the solution.
• Good agents -Bleach, Radiact wash, Am4OH
• Clean till no signal
• RPO will do wipe test
• If still signal cover till decayed.

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Biological

• BL1- E. Coli
• low BL2 - Salmonella typhurminium LT2
• Clean up
• All bacteria needs to be in activated by before
  disposal or going to the kitchen
• Autoclaving
• Chemical, 10 bleach, 1 Westcodyne, Lysol
• Waste
• Plates go into the bucket autoclaved in big
  autoclave after kitchen hours.

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Biological spills

• Block the area
• Let aerosol settle
• Safety gear
• Clean up by pour/spray disinfectant on glassware
• with dusty pan
• Put paper towel on it and pour/spray disinfectant
  on it
• Dispose of in the waste.
• Look for the new biological spill kits

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Decontaminating the Bench

• 70 ethanol is a better agent to decontaminate
  the bench than 100 ethanol
• 70 is better at denaturing the outer membrane
  and subsequently lysing the cell
• 100 will only causes effects the on the outer
  membrane but not lysis.

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High Voltage

• Any voltage should be treated as if it is
  dangerous.
• AC current is the most dangerous.
• Running a PAGE
• Start up a gel by connecting the black lead first
  as a ground and then the red lead.
• It should be noted that high power leads should
  be used when running a sequencing gel with high
  voltage.
• Turn off a power supply by turning down the
  voltage source, then turn off the power.
• After turning off power, disconnect the red
  (positive) lead first.
• If the hazard light is flashing on the power
  supply, turn it off and determine what the
  problem is and fix it, or have it fixed.
• If someone is unconscious, call x100 which is the
  emergency phone number at MIT.

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Chemical Hygiene Plan

• OSHA right to know
• MSDS know how to read
• SOP- Standard Operating Procedures
• a written procedure for use of every chemical

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Safety is everyone responsibility

• Everyone will be responsible for the level 1
  check list which I email the pdf
• keep check off for a month
• Cammie and Shannon are the safety Reps to do an
  over view
• ensure all unlabeled liquid containing item have
  a label can be cryptic