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TEXAS AM INSTITUTE FOR GENOMIC MEDICINe
Knockout Resources and Services for Contract RD
Jennifer DAngelo, MBA Business Development
Marketing Manager Texas AM Institute for Genomic
Medicine jdangelo_at_tigm.org
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Overview

• About Us
• Facilities
• Gene Trap Technology
• Resources
• Quality Control
• Online Ordering/Database
• Services
• Terms and Conditions
• Texas AM Capabilities Contract RD

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About TIGM

• Originally created by the State of Texas as a
  non-profit independent organization
• Joint institute of Texas AM University and Texas
  AM Health Science Center
• Operates the worlds largest gene trap library to
  create knockout mice
• Provides a variety of mouse model research
  services for our own knockout models as well as
  mouse models from other institutions

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Our Mission

• Discover breakthroughs in science and medicine
  and accelerate the pace of medical discoveries
  through internal research and by providing our
  resources and services to the scientific community

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Headquarters and Research LabsHouston, Texas

• Located in the Texas Medical Center

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Mouse FacilityCollege Station, Texas

• Capacity for 80,000 genetically engineered mice
• Full shower-in barrier including animal, surgery,
  necropsy, procedure, and neurobehavioral
  phenotyping rooms.

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TIGM Gene Trap

• Retroviral insertion of gene trapping vector
  containing a promoter-less marker/reporter gene
  (Neo, or ß-Geo)

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C57BL/6 Library Construction
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C57BL/6N Gene Trap Library

• Worlds largest C57BL/6 knockout mouse library
• gt350,000 ES cell clones
• gt10,000 unique genes

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Additional TIGM Resources

• Access to 129/SvEvBrd Gene Trap and Gene Targeted
  Library
• over 272,000 ES cell clones
• more than 9,000 unique genes

Combined total of over 640,000 clones containing
over 13,000 unique genes
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Quality Control
Quality control procedures for ES cell clones

• qPCR for Neo to ensure one copy of gene trapping
  vector is present in the genome
• qPCR for SRY to ensure there is no loss or
  degradation of the Y chromosome
• iPCR to ensure the gene trapping vector is in the
  correct location and orientation
• Thioglycolate and PCR assays to monitor yeast,
  mold, bacteria and Mycoplasma contamination

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Quality Control
Quality control procedures for mice

• Clones undergo quality control procedures before
  they are microinjected
• Tail samples are genotyped with wild type and
  mutant strategies specific for the insertion site
  before the animals are shipped

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Online Database/Ordering

• TIGM has an extensive, user-friendly database
  accessible via the Internet. It allows you to
  search through our C57BL/6 or the 129SvEvBrd gene
  trap libraries, inquire about a specific line,
  and place an order.
• You can search by
• Entrez Gene ID
• Gene symbol
• Keyword
• Sequence in FASTA format
• Chromosome

www.tigm.org/database
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Online Database/Ordering
TIGMs database links directly to the UCSC Genome
Browser and allows you to view the sequence tags
generated from our vector insertion sites. Click
the TIGM KNOCKOUTS link at the top of the UCSC
genome browser window to view all of TIGMs
available clones.
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TIGM Services
TIGM offers many services beyond creating
knockout lines. We offer these services for mice
that are generated from the TIGM library as well
as mice obtained from other organizations.

• Custom projects with homologous recombination
• Blastocyst injection
• Pronuclear microinjection
• Rederivation of transgenic lines
• Embryo transfer
• Colony maintenance
• Colony expansion

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TIGM Services - Phenotyping

• Clinical Chemistry
• Albumin
• Bilirubin
• Cholesterol
• Electrolytes (Na, K, Cl, Mg)
• Glucose
• Etc
• Hematology
• Hemoglobin
• White blood count (WBC)
• Serum folate
• Etc

• Pathology/Histology
• Bone Marrow (Femoral)
• Cerebrum
• Lymph Node
• Nerve (Sciatic)
• Kidneys
• Liver
• Aorta
• Etc
• To view more phenotyping services, please visit
• http//tigm.org/PhenotypeAnalysis20080326.pdf
• Or click Other TIGM services from the Access
  page on our website.

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Terms and Conditions

• Resources available through a simple MTA
• TIGMs resources may not be sold or redistributed
  to other entities.
• All discoveries made with TIGM mice are the
  property of the researcher with no reach through
  claims on any IP generated

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Texas AM System Academic Research Basic and
Translational

• TIGM
• Target Identification
• Initial Validation
• GLP ADME/Tox
• GLP Small Animal Testing

• TIPS
• Large Animal Model development
• GLP ADME/Tox
• GLP Large Animal Testing

• GMP Therapeutics Manufacturing
• Initial Material Production
• Material Scale-up
• Formulation/Delivery

Texas AM
Health Science Center and Clinical Trial Centers
(Phase I IIA)
Academic Research Collaborations Basic and
Clinical Research
Federal State Initiatives Vaccines, Biologics
and Small Molecules
Pharmaceutical Companies Discovery RD, GLP, HTS
and GMP
Biotechnology Companies Discovery RD, GLP, HTS
and GMP
Medical Device Companies Device Design,
Prototyping and GLP
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Outsource Mouse Model RD
Resources
Facilities
System
Knowledge Experience
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Easy Access to TIGM Resources Services

• Web
• 24/7 www.tigm.org
• Phone
• 1-713-677-7429
• 888-377-TIGM (8446)
• 9 am - 4 pm (Central Time)
• Email
• info_at_tigm.org